Roles of miR-4442 in Colorectal Cancer: Predicting Early Recurrence and Regulating Epithelial-Mesenchymal Transition

被引：5

作者：

Shibamoto, Jun ^{[1
]}

Arita, Tomohiro ^{[1
]}

Konishi, Hirotaka ^{[1
]}

Kataoka, Satoshi ^{[1
]}

Furuke, Hirotaka ^{[1
]}

Takaki, Wataru ^{[1
]}

Kiuchi, Jun ^{[1
]}

Shimizu, Hiroki ^{[1
]}

Yamamoto, Yusuke ^{[1
]}

Komatsu, Shuhei ^{[1
]}

Shiozaki, Atsushi ^{[1
]}

Kuriu, Yoshiaki ^{[1
]}

Otsuji, Eigo ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Dept Surg, Div Digest Surg, 465 Kajii-cho, Kawaramachi Hirokoji, Kamigyo Ku, Kyoto 6028566, Japan

来源：

GENES | 2023年 / 14卷 / 07期

关键词：

biomarker; colorectal cancer; epithelial-mesenchymal transition; microRNA; recurrence; EARLY RELAPSE; CURATIVE SURGERY; PROGNOSIS; MICRORNAS; PROGRESSION; INHIBITION; ANTIGEN; GROWTH;

D O I：

10.3390/genes14071414

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Early recurrence in patients with colorectal cancer (CRC) is associated with a poor prognosis. We aimed to identify circulating microRNAs that are biomarkers of early CRC recurrence and elucidate their functions. We identified miR-4442 as a candidate biomarker by microRNA array analysis comparing preoperative and postoperative plasma levels in patients with CRC, with and without recurrence. The association between preoperative plasma miR-4442 levels, clinicopathological features, and recurrence-free survival was analyzed in 108 patients with CRC after curative surgery. Furthermore, cell-function analyses were performed, and the involvement of miR-4442 in regulating epithelial-mesenchymal transition (EMT) was examined. Preoperatively plasma miR-4442 levels were associated with CRC recurrence and exhibited an incremental increase with earlier recurrence dates. Moreover, miR-4442 demonstrated high sensitivity and specificity as a potential biomarker for early CRC recurrence. The expression of miR-4442 in cancer tissues of patients with metastatic liver cancer from CRC was higher than in normal liver, CRC, and normal colorectal tissues. The overexpression of miR-4442 promoted the proliferative, migratory, and invasive activities of CRC cells, decreased levels of RBMS1 and E-cadherin, and increased levels of N-cadherin and Snail1. Plasma miR-4442 is a clinically useful biomarker for predicting the early recurrence of CRC. Furthermore, miR-4442 regulates EMT in CRC by directly targeting the messenger RNA of RBMS1.

引用

页数：18

共 54 条

[41] miR-6858 plays a key role in the process of melatonin inhibition of the malignant biological behavior of glioma [J].