G-quadruplex-hemin DNAzyme functionalized nanopipettes: Fabrication and sensing application

被引：10

作者：

Dong, Jingyi ^{[1
]}

Qiu, Xia ^{[1
]}

Huang, Mimi ^{[1
]}

Chen, Xiaohu ^{[1
]}

Li, Yongxin ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Chem & Mat Sci, Key Lab Funct Mol Solids, Anhui Key Lab Chemo Biosensing,Minist Educ, Wuhu 241000, Peoples R China

来源：

TALANTA | 2023年 / 257卷

基金：

中国国家自然科学基金;

关键词：

Nanopipette; G-quadruplex-hemin DNAzyme; Ion current; Hydrogen peroxide; HYDROGEN-PEROXIDE; ELECTROCHEMICAL DETECTION; CURRENT RECTIFICATION; GLASS NANOPORE; DNA; MOLECULE; CONVERSION; PROTEINS; CANCER; H2O2;

D O I：

10.1016/j.talanta.2023.124384

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Solid-nanopores/nanopipettes have the exquisite ability to reveal the changes in molecular volume due to the advantages of adjustable size, good rigidity and low noise. Herein, a new platform for sensing application was established based on G-quadruplex-hemin DNAzyme (GQH) functionalized gold-coated nanopipettes. In this method, GQH was immobilized on gold-coated nanopipette, which could be used as a catalyst for the reaction of H2O2 with 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to promote the conversion of ABTS to ABTS+ ions inside gold-coated nanopipette, and the change of transmembrane ion current could be monitored in real time. At the optimal conditions, there was a correlation between the ion current and the concentration of H2O2 in a certain range, which could be used for the hydrogen peroxide sensing. The GQH immobilized nanopipette provides a useful platform to investigate enzymatic catalysis in confined environment, which can be used in electrocatalysis, sensing and fundamental electrochemistry.

引用

页数：7

共 62 条

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