Cannabidiol Rescues TNF-α-Inhibited Proliferation, Migration, and Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells

被引:15
作者
Yu, Lina [1 ,2 ]
Zeng, Liting [1 ,2 ]
Zhang, Zeyu [1 ,2 ]
Zhu, Guanxiong [1 ,2 ]
Xu, Zidan [1 ,2 ]
Xia, Junyi [1 ,2 ]
Weng, Jinlong [1 ,2 ]
Li, Jiang [1 ,2 ]
Pathak, Janak Lal [1 ]
机构
[1] Guangzhou Med Univ, Guangdong Engn Res Ctr Oral Restorat & Reconstruct, Dept Prevent Dent, Guangzhou Key Lab Basic & Appl Res Oral Regenerat, Guangzhou 510182, Peoples R China
[2] Guangzhou Med Univ, Sch & Hosp Stomatol, Guangzhou 510182, Peoples R China
基金
中国国家自然科学基金; 英国科研创新办公室;
关键词
pulpitis; dental pulp stem cells; dentin; pulp regeneration; cannabidiol; inflammation; CANNABINOIDS; RESPONSES; MODEL;
D O I
10.3390/biom13010118
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Strategies to promote dental pulp stem cells (DPSCs) functions including proliferation, migration, pro-angiogenic effects, and odontogenic/osteogenic differentiation are in urgent need to restore pulpitis-damaged dentin/pulp regeneration and DPSCs-based bone tissue engineering applications. Cannabidiol (CBD), an active component of Cannabis sativa has shown anti-inflammation, chemotactic, anti-microbial, and tissue regenerative potentials. Based on these facts, this study aimed to analyze the effect of CBD on DPSCs proliferation, migration, and osteogenic/odontogenic differentiation in basal and inflammatory conditions. Highly pure DPSCs with characteristics of mesenchymal stem cells (MSCs) were successfully isolated, as indicated by the results of flowcytometry and multi-lineage (osteogenic, adipogenic, and chondrogenic) differentiation potentials. Among the concentration tested (0.1-12.5 mu M), CBD (2.5 mu M) showed the highest anabolic effect on the proliferation and osteogenic/odontogenic differentiation of DPSCs. Pro-angiogenic growth factor VEGF mRNA expression was robustly higher in CBD-treated DPSCs. CBD also prompted the migration of DPSCs and CBD receptor CB1 and CB2 expression in DPSCs. TNF-alpha inhibited the viability, migration, and osteogenic/odontogenic differentiation of DPSCs and CBD reversed these effects. CBD alleviated the TNF-alpha-upregulated expression of pro-inflammatory cytokines TNF-alpha, interleukin (IL)-1 beta, and IL-6 in DPSCs. In conclusion, our results indicate the possible application of CBD on DPSCs-based dentin/pulp and bone regeneration.
引用
收藏
页数:17
相关论文
共 50 条
  • [41] Osteogenic differentiation of dental pulp stem cells under the influence of three different materials
    Ajlan, Sumaiah A.
    Ashri, Nahid Y.
    Aldahmash, Abdullah M.
    Alnbaheen, May S.
    BMC ORAL HEALTH, 2015, 15
  • [42] The Role of Interleukin 6 in Osteogenic and Neurogenic Differentiation Potentials of Dental Pulp Stem Cells
    Park, Yong-Tae
    Lee, Su-Min
    Kou, Xiaoxing
    Karabucak, Bekir
    JOURNAL OF ENDODONTICS, 2019, 45 (11) : 1342 - 1348
  • [43] Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway
    Jing Na
    Lingyu Zhang
    Lisha Zheng
    Jingyi Jiang
    Qiusheng Shi
    Chiyu Li
    Yubo Fan
    Cytotechnology, 2022, 74 : 395 - 405
  • [44] Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway
    Na, Jing
    Zhang, Lingyu
    Zheng, Lisha
    Jiang, Jingyi
    Shi, Qiusheng
    Li, Chiyu
    Fan, Yubo
    CYTOTECHNOLOGY, 2022, 74 (03) : 395 - 405
  • [45] Behaviour of human dental pulp stem cell in high glucose condition: impact on proliferation and osteogenic differentiation
    Kichenbrand, Charlene
    Grossin, Laurent
    Menu, Patrick
    Moby, Vanessa
    ARCHIVES OF ORAL BIOLOGY, 2020, 118
  • [46] TNF-α, IL-1B and IL-6 affect the differentiation ability of dental pulp stem cells
    Sema Sonmez Kaplan
    Hesna Sazak Ovecoglu
    Deniz Genc
    Tunc Akkoc
    BMC Oral Health, 23
  • [47] TNF-α, IL-1B and IL-6 affect the differentiation ability of dental pulp stem cells
    Kaplan, Sema Sonmez
    Ovecoglu, Hesna Sazak
    Genc, Deniz
    Akkoc, Tunc
    BMC ORAL HEALTH, 2023, 23 (01)
  • [48] Low Dose Sophora flavescens Polysaccharide Promote the Migration and Osteogenic Differentiation of Dental Pulp Stem Cells and Up-Regulate the Expression of Osteogenic Genes
    Yao, W. E. N. X. I. A.
    Zheng, X. I. A. O. Q. I. A. N.
    Pu, Y. I. N. G. X. U. A. N.
    Yasen, M.
    INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2023, 85 : 32 - 36
  • [49] Improved osteogenic differentiation of human dental pulp stem cells in a layer-by-layer-modified gelatin scaffold
    Fu, Qiang
    Ren, Huaijuan
    Zheng, Chen
    Zhuang, Chao
    Wu, Tong
    Qin, Jinyan
    Wang, Zejian
    Chen, Yantian
    Qi, Nianmin
    JOURNAL OF BIOMATERIALS APPLICATIONS, 2018, 33 (04) : 477 - 487
  • [50] Calcium Hydroxide-induced Proliferation, Migration, Osteogenic Differentiation, and Mineralization via the Mitogen-activated Protein Kinase Pathway in Human Dental Pulp Stem Cells
    Chen, Luoping
    Zheng, Lisha
    Jiang, Jingyi
    Gui, Jinpeng
    Zhang, Lingyu
    Huang, Yan
    Chen, Xiaofang
    Ji, Jing
    Fan, Yubo
    JOURNAL OF ENDODONTICS, 2016, 42 (09) : 1355 - 1361