Circulating miR-206, miR-181b, and miR-21 as promising biomarkers in hypothyroidism and their relationship to related hyperlipidemia and hepatic steatosis

被引:1
作者
Mohammed, Asmaa [1 ]
Shaker, Olfat G. [2 ]
Khalil, Mahmoud A. F. [3 ]
Abu-El-Azayem, Abeer K. [4 ]
Samy, Amira [5 ]
Fathy, Shaimaa A. [6 ]
AbdElguaad, Mohamed M. K. [7 ]
Mahmoud, Fatma A. M. [8 ]
Erfan, Randa [2 ]
机构
[1] Fayoum Univ, Fac Med, Dept Med Biochem & Mol Biol, Al Fayyum, Egypt
[2] Cairo Univ, Fac Med, Dept Med Biochem & Mol Biol, Cairo, Egypt
[3] Fayoum Univ, Fac Pharm, Dept Microbiol & Immunol, Al Fayyum, Egypt
[4] Cairo Univ, Fac Med, Dept Med Microbiol & Immunol, Cairo, Egypt
[5] Cairo Univ, Fac Med, Dept Clin & Chem Pathol, Cairo, Egypt
[6] Cairo Univ, Fac Med, Dept Internal Med Diabet & Endocrinol, Cairo, Egypt
[7] Fayoum Univ, Fac Med, Dept Physiol, Al Fayyum, Egypt
[8] Fayoum Univ, Fac Med, Dept Trop Med, Al Fayyum, Egypt
关键词
thyroid hormone; hepatic steatosis; microRNA; miR-206; MiR-181b; miR-21; LIVER STIFFNESS MEASUREMENT; RECURRENCE; MICRORNAS; DISEASE;
D O I
10.3389/fmolb.2024.1307512
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Thyroid hormones (THs) signaling has profound effects on many physiological processes. The regulation of THs signaling in various tissues involves the action of microRNAs (miRNAs) on thyroid deiodinases and receptors. THs regulate the expression of certain miRNAs and their target messenger RNAs (mRNAs) in various tissues and cells. The modulation of miRNA levels by THs affects their functions in processes such as liver lipid metabolism, skin physiology, and muscle and heart performance.Aim: This research aimed to investigate miR-181b, miR-206, and miR-21 in the serum of patients with subclinical and overt hypothyroidism to determine their possible role in the diagnosis of the disease and their relationship to clinical disorders related to hypothyroidism.Methods: This study included ninety participants, divided evenly into three groups as follows: patients with overt hypothyroidism diagnosed clinically, radiologically, and by investigation, subclinical hypothyroid patients, and healthy volunteers. The patients had a thorough medical history and underwent a clinical examination. Laboratory tests included plasma cholesterol, LDL, HDL, TGs, liver and renal function tests, CBC, fasting insulin, HOMA-IR, HbA1c, TSH, and free T4. The serum levels of miR-21, miR-206, and miR-181b were measured using qRT-PCR.Results: miR-206 and miR-181b levels were higher in the subclinical group, followed by the hypothyroid and control groups. For miR-21, there was a significantly lower mean value in both the hypothyroid and subclinical groups than in the control group, with no difference between the two groups. Both miR-206 and miR-181b showed a significant negative association with albumin and free T4 levels and a significant direct association with GGT, ALT, AST, creatinine, uric acid, TGs, TC, LDL, TSH, thyroid volume, and CAP score. The same correlation pattern was observed for miR-181b, except that it was not significantly correlated with the TGs. For miR-21 levels, there was a significant positive correlation with albumin, free T4 level, and kPa score and a negative correlation with GGT, ALT, AST, creatinine, uric acid, HOMA-IR, HbA1c, TC, LDL, TSH, and CAP score. Cases with F1 kPa score and S2 CAP scores had significantly higher averages for miR-206 and miR-181b, with a p-value of 0.05. Moreover, miR-21 levels were significantly lower in the S2 CAP score group.Conclusion: These miRNAs (miR-206, miR-181b, and miR-21) may be used as diagnostic biomarkers for hypothyroidism. They may be used as therapeutic targets to control dyslipidemia and hepatic steatosis during hypothyroid disease.
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页数:14
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共 44 条
  • [1] Ammar A., 2021, World J. Adv. Res. Rev, V12, P275, DOI [10.30574/wjarr.2021.12.1.0477, DOI 10.30574/WJARR.2021.12.1.0477]
  • [2] MicroRNAs and thyroid hormone action
    Aranda, Ana
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2021, 525
  • [3] Possible use of miRNAs-146a and-499 expression and their polymorphisms as diagnostic markers for rheumatoid arthritis
    Ayeldeen, Ghada
    Nassar, Yasser
    Ahmed, Hanan
    Shaker, Olfat
    Gheita, Tamer
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2018, 449 (1-2) : 145 - 156
  • [4] MicroRNAs: Target Recognition and Regulatory Functions
    Bartel, David P.
    [J]. CELL, 2009, 136 (02) : 215 - 233
  • [5] Comparison of BD Vacutainer SST™ Plus Tubes with BD SST™ II Plus Tubes for common analytes
    Bush, VJ
    Janu, MR
    Bathur, F
    Wells, A
    Dasgupta, A
    [J]. CLINICA CHIMICA ACTA, 2001, 306 (1-2) : 139 - 143
  • [6] Pitfalls of Liver Stiffness Measurement: A 5-Year Prospective Study of 13,369 Examinations
    Castera, Laurent
    Foucher, Juliette
    Bernard, Pierre-Henri
    Carvalho, Francoise
    Allaix, Daniele
    Merrouche, Wassil
    Couzigou, Patrice
    de Ledinghen, Victor
    [J]. HEPATOLOGY, 2010, 51 (03) : 828 - 835
  • [7] Hypothyroidism
    Chaker, Layal
    Bianco, Antonio C.
    Jonklaas, Jacqueline
    Peeters, Robin P.
    [J]. LANCET, 2017, 390 (10101) : 1550 - 1562
  • [8] Loss of DNA methylation is related to increased expression of miR-21 and miR-146b in papillary thyroid carcinoma
    Dias Payao Ortiz, Isabella Maria
    Barros-Filho, Mateus Camargo
    dos Reis, Mariana Bisarro
    Beltrami, Caroline Moraes
    Marchi, Fabio Albuquerque
    Kuasne, Hellen
    do Canto, Luisa Matos
    Homem de Mello, Julia Bette
    Abildgaard, Cecilie
    Lopes Pinto, Clovis Antonio
    Kowalski, Luiz Paulo
    Rogatto, Silvia Regina
    [J]. CLINICAL EPIGENETICS, 2018, 10
  • [9] Thyroid Hormone May Regulate mRNA Abundance in Liver by Acting on MicroRNAs
    Dong, Hongyan
    Paquette, Martin
    Williams, Andrew
    Zoeller, R. Thomas
    Wade, Mike
    Yauk, Carole
    [J]. PLOS ONE, 2010, 5 (08):
  • [10] Analysis of risk factors for the development of type 2 diabetes mellitus complicated with Hashimoto's thyroiditis
    Han, Meng
    Wu, Haoneng
    Yang, Weiming
    Chen, Juanjuan
    [J]. BMC ENDOCRINE DISORDERS, 2022, 22 (01)