Bovine Serum Albumin (BSA) Mediated Efficient One Pot Synthesis of 3,3′-(Phenylmethanediyl)bis(4-hydroxy-6-methyl-2H-pyran-2-one) Derivatives and Their Biological Studies

In this work, biocatalysis was achieved by the synthesis of 3,3 ' -(phenylmethanediyl)bis(4-hydroxy6-methyl-2H-pyran-2-one) derivatives (IIIa-IIIh) using Bovine Serum Albumin (BSA) as a catalyst. Substrate scope and various parameters involved on yield and time of the synthesis were investigated. The structure elucidation of the synthesised targets was assessed through different spectroscopic techniques like FT-IR, H-1 NMR,C-13 NMR, and HR-MS analysis. Bio potency of the synthesised conjugates was examined towards anti-bacterial activity and most of the compounds showed effective results. Further, the anti-inflammatory activity of the synthesised compounds was evaluated against MMP-2 and MMP-9 using gelatine zymography. All the synthesised derivatives inhibited MMP-2 to good extent but displayed a moderate inhibition against MMP-9. Also the molecular docking studies were carried out to determine the interaction of synthesised bis-4-hydroxy-6-methyl-pyran-2-onederivatives with COX-2 receptor.