Curcumin-primed periodontal ligament stem cells-derived extracellular vesicles improve osteogenic ability through the Wnt/β-catenin pathway

被引：11
作者：
Lan, Qian [1 ]
Cao, Jiadong [1 ]
Bi, Xueting [1 ]
Xiao, Xin [1 ]
Li, Dongsheng [2 ]
Ai, Yilong [1 ]
机构：
[1] Foshan Univ, Foshan Stomatol Hosp, Sch Med, Foshan, Guangdong, Peoples R China
[2] Deja Lab, Foshan, Guangdong, Peoples R China
来源：
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2023年 / 11卷
关键词：
curcumin; extracellular vesicles; periodontal ligament stem cells; oteogenesis; Wnt/beta-catenin; EXOSOMES; DENSITY;
D O I：
10.3389/fcell.2023.1225449
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Introduction: Curcumin has broad application prospects in the prevention and treatment of periodontal diseases. Periodontal ligament stem cell-derived extracellular vesicles (PDLSC-EV) can effectively promote periodontal tissue regeneration and possess good drug delivery capability. Superior pharmacological effects can be exerted using PDLSC-EV as a curcumin carrier.Methods: In the present study, we constructed curcumin-primed PDLSCs-derived extracellular vesicles (Cur-PDLSC-EV) from cell culture supernatants of curcumin-pretreated PDLSCs by ultracentrifugation and investigated their effects on the proliferation, migration, and osteogenic ability of PDLSCs and the corresponding downstream molecular pathways.Results: Both Cur-PDLSC-EV and PDLSC-EV promoted osteoblast proliferation and migration. Compared with PDLSC-EV, Cur-PDLSC-EV possessed a more potent pro-osteogenic ability. Moreover, the improved osteogenesis of Cur-PDLSC-EV was related to the activation of the Wnt/beta-catenin signaling pathway.Conclusion: This study suggests that Cur-PDLSC-EV can promote osteogenic differentiation by activating Wnt/beta-catenin, providing reference bases for the treatment of periodontal diseases.
引用
收藏
页数：9
相关论文
共 39 条
[11]   Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells [J].
Iwasaki, Kengo ;
Akazawa, Keiko ;
Nagata, Mizuki ;
Komaki, Motohiro ;
Peng, Yihao ;
Umeda, Makoto ;
Watabe, Tetsuro ;
Morita, Ikuo .
DENTISTRY JOURNAL, 2021, 9 (01)
[12]   Curcumin-primed and curcumin-loaded exosomes: potential neural therapy [J].
Kalani, Anuradha ;
Chaturvedi, Pankaj .
NEURAL REGENERATION RESEARCH, 2017, 12 (02) :205-206
[13]   Lipopolysaccharide-Preconditioned Periodontal Ligament Stem Cells Induce M1 Polarization of Macrophages through Extracellular Vesicles [J].
Kang, Hyejong ;
Lee, Myung-Ju ;
Park, Sang June ;
Lee, Myung-Shin .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (12)
[14]   Mesenchymal stem cell-derived extracellular vesicles: novel frontiers in regenerative medicine [J].
Keshtkar, Somayeh ;
Azarpira, Negar ;
Ghahremani, Mohammad Hossein .
STEM CELL RESEARCH & THERAPY, 2018, 9
[15]   Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects [J].
Kotha, Raghavendhar R. ;
Luthria, Devanand L. .
MOLECULES, 2019, 24 (16)
[16]   Effects of periodontal ligament stem cell-derived exosomes on osteoblastic proliferation, migration, differentiation, apoptosis, and signaling pathways [J].
Lan, Qian ;
Xiao, Xin ;
Bi, Xueting ;
Gu, Yangcong ;
Ai, Yilong .
ORAL DISEASES, 2024, 30 (02) :710-718
[17]   Treatment of inflammatory bone loss in periodontitis by stem cell-derived exosomes [J].
Lei, Fengzhen ;
Li, Mujia ;
Lin, Tingting ;
Zhou, Hong ;
Wang, Fei ;
Su, Xiaoxia .
ACTA BIOMATERIALIA, 2022, 141 :333-343
[18]   Inflammasomes in Alveolar Bone Loss [J].
Li, Yang ;
Ling, Junqi ;
Jiang, Qianzhou .
FRONTIERS IN IMMUNOLOGY, 2021, 12
[19]   Mesenchymal stem cells, exosomes and exosome-mimics as smart drug carriers for targeted cancer therapy [J].
Liu, Hongmei ;
Deng, Shichen ;
Han, Lu ;
Ren, Yan ;
Gu, Jian ;
He, Lili ;
Liu, Tianqing ;
Yuan, Zhi-xiang .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2022, 209
[20]   Human Periodontal Ligament Stem Cell-Derived Exosomes Promote Bone Regeneration by Altering MicroRNA Profiles [J].
Liu, Ting ;
Hu, Wenyun ;
Zou, Xue ;
Xu, Jingchen ;
He, Shushu ;
Chang, Le ;
Li, Xinyi ;
Yin, Yuanyuan ;
Tian, Mi ;
Li, Ziyu ;
Zhou, Jialiang ;
Jiang, Xiaoge ;
Chen, Song .
STEM CELLS INTERNATIONAL, 2020, 2020
1234