Integrin α6 Targeted Near Infrared Fluorescent Imaging and Photoacoustic Imaging of Hepatocellular Carcinoma in Mice

被引：4

作者：

Lin, Yan-Zhu ^{[1
]}

Wu, You ^{[1
]}

Cao, De-Hai ^{[1
]}

Peng, Yong-Jian ^{[1
]}

Deng, Jun ^{[2
]}

Lin, Wen-Jie ^{[1
]}

Si-Tu, Min-Yi ^{[1
]}

Zhuo, Ling ^{[1
]}

Chen, Jie-Min ^{[1
]}

Lei, Man-Xia ^{[3
]}

Liu, Rong-Bin ^{[4
]}

Zhang, Wei-Guang ^{[1
]}

Li, Jian-Jun ^{[1
]}

Yang, Xiao-Chun ^{[1
]}

Feng, Guo-Kai ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China

[2] Guangdong Inst Drug Control, Dept Biol Prod, Guangzhou, Guangdong, Peoples R China

[3] Southern Med Univ, Nanfang Hosp, Dept Endocrinol, Guangzhou, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Ultrasound, Guangzhou, Guangdong, Peoples R China

来源：

JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY | 2023年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Photoacoustic imaging; Near-infrared fluorescent imaging; Integrin alpha 6; Peptide; Molecular imaging; Hepatocellular carcinoma; IN-VIVO; PEPTIDE; DELIVERY;

D O I：

10.14218/JCTH.2021.00414

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background and Aims: Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer-related death and ranks sixth in terms of incident cases worldwide. The purpose of this study was to develop an effective and sensitive method to distinguish liver cancer tissues from normal tissues in HCC patients. Integrin alpha 6 is a promising cell surface target for molecular imaging of HCC, where it is overexpressed and is a prognostic biomarker. We previously identified an integrin alpha 6-targeted peptide CRWYDENAC (RWY) that has been used for positron emission tomography (PET) imaging of HCC in mouse models. Methods: We labeled the integrin a6-targeted RWY peptide with cyanine 7 (Cy7) to form an optical probe (Cy7-RWY) for near infrared fluorescent (NIRF) and photoacoustic (PA) imaging in HCC. Mice transplanted with subcutaneous HCC-LM3 or orthotopic HCC-H22 cells that overexpressed integrin a6 were intravenously injected with Cy7-RWY and its corresponding Cy7-control. NIRF and PA images of mice were collected from 0 to 48 h after injection. Results: Both NIRF and PA signals started to accumulate in the tumor 2 h after injection of Cy7-RWY and peaked at 24 h. Conclusions: Cy7-RWY is a promising optical probe for NIRF and PA imaging of HCC in mice, and has potential clinical application for HCC detection.

引用

页码：110 / 117

页数：8

共 37 条

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