Biosynthesis of Peptide Hydrogel-Titania Nanoparticle Composites with Antibacterial Properties

被引：4

作者：

Binaymotlagh, Roya ^{[1
]}

Haghighi, Farid Hajareh ^{[1
]}

Di Domenico, Enea Gino ^{[2
]}

Sivori, Francesca ^{[3
]}

Truglio, Mauro ^{[3
]}

Del Giudice, Alessandra ^{[1
]}

Fratoddi, Ilaria ^{[1
]}

Chronopoulou, Laura ^{[1
,4
]}

Palocci, Cleofe ^{[1
,4
]}

机构：

[1] Sapienza Univ Rome, Dept Chem, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[2] Sapienza Univ Rome, Dept Biol & Biotechnol C Darwin, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[3] San Gallicano Dermatol Inst, Microbiol & Virol, IRCCS, I-00144 Rome, Italy

[4] Sapienza Univ Rome, Res Ctr Appl Sci Safeguard Environm & Cultural Her, CIABC, Piazzale Aldo Moro 5, I-00185 Rome, Italy

来源：

GELS | 2023年 / 9卷 / 12期

关键词：

peptide-based hydrogels; titania nanoparticles; antibacterial properties; hydrogel composites; Staphylococcus aureus; MRSA; skin; NANOCOMPOSITES;

D O I：

10.3390/gels9120940

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The photoantibacterial properties of titania nanoparticles (TiO(2)NPs) are attracting much interest, but the separation of their suspension limits their application. In this study, the encapsulation of commercial TiO(2)NPs within self-assembling tripeptide hydrogels to form hgel-TiO2NP composites with significant photoantibacterial properties is reported. The Fmoc-Phe(3) hydrogelator was synthesized via an enzymatic method. The resulting composite was characterized with DLS, zeta-potential, SAXS, FESEM-EDS and rheological measurements. Two different concentrations of TiO(2)NPs were used. The results showed that, by increasing the TiO2NP quantity from 5 to 10 mg, the value of the elastic modulus doubled, while the swelling ratio decreased from 63.6 to 45.5%. The antimicrobial efficacy of hgel-TiO(2)NPs was tested against a laboratory Staphylococcus aureus (S. aureus) strain and two methicillin-resistant S. aureus (MRSA) clinical isolates. Results highlighted a concentration-dependent superior antibacterial activity of hgel-TiO(2)NPs over TiO(2)NPs in the dark and after UV photoactivation. Notably, UV light exposure substantially increased the biocidal action of hgel-TiO(2)NPs compared to TiO(2)NPs. Surprisingly, in the absence of UV light, both composites significantly increased S. aureus growth relative to control groups. These findings support the role of hgel-TiO(2)NPs as promising biocidal agents in clinical and sanitation contexts. However, they also signal concerns about TiO2NP exposure influencing S. aureus virulence.

引用

页数：16