Simultaneous detection of peroxynitrite and viscosity in diabetes by a dual response fluorescent probe

被引：17

作者：

Zhang, Jinzheng ^{[1
]}

Gong, Shenyi ^{[1
]}

Liu, Yijia ^{[1
]}

Zheng, Zhoupeng ^{[1
]}

Feng, Guoqiang ^{[1
]}

机构：

[1] Cent China Normal Univ, Coll Chem, 152 Luoyu Rd, Wuhan 430079, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2024年 / 406卷

基金：

中国国家自然科学基金;

关键词：

Dual -channel fluorescent probe; Viscosity; Peroxynitrite; Mitochondria; Diabetes; In vivo imaging; MITOCHONDRIAL DYSFUNCTION; LIVING CELLS;

D O I：

10.1016/j.snb.2024.135432

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Diabetes is a common metabolic disease worldwide, which often has serious complications and seriously threatens human health. Abnormal viscosity and peroxynitrite (ONOO-) concentration are closely related to diabetes. Herein, we report a fluorescent dual response probe AO, which can simultaneously detect the changes of viscosity and ONOO- in diabetes at dual fluorescence channels. AO sensitively responds to viscosity and ONOO- with significant emission signal changes at 710 nm and 645 nm, respectively. Moreover, AO selectively targets mitochondria and generates a new compound when detecting ONOO-, which can spontaneously target and light up another type of organelle (lipid droplets), thus showing spatially separated dual -channel fluorescence signals. More notably, AO was successfully used to simultaneously detect the viscosity and ONOO- variations in hyperglycemic cells, diabetes and its complications (using mice models) for the first time, which may lead to a better understanding of the physiological and pathological phenomena of diabetes.

引用

页数：9