Newly isolated Brevundimonas naejangsanensis as a biocontrol agent against Fusarium redolens the causal of Fusarium yellows of chickpea

被引:2
作者
Bekkar, Ahmed Amine [1 ]
Zaim, Souad [1 ]
机构
[1] Univ Mustapha Stambouli Mascara, Fac Life & Nat Sci, Dept Agron, Lab Res Biol Syst & Geomat LRSBG, Mascara, Algeria
关键词
Biocontrol; Fusarium redolens; Brevundimonas naejangsanensis; Antagonistic activities; Plant growth promoting; GROWTH PROMOTION; BIOLOGICAL-CONTROL; GENETIC DIVERSITY; CO-INOCULATION; ANTAGONISTS; BACTERIA; WILT; ROT;
D O I
10.1007/s12223-023-01126-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Three endophytic bacteria, namely BvV, BvP and BvL, were newly isolated from the root nodules of bean, pea and lentil plants respectively cultivated in Mascara the northwest of Algeria, and identified by 16S ribosomal RNA gene sequencing as Brevundimonas naejangsanensis. These strains were able to produce hydrolytic enzymes and hydrogen cyanide. All strains produced a growth-promoting hormone, indole acetic acid, varying in concentration from 83.2 to 171.7 mu g/mL. The phosphate solubilizing activity of BvV, BvP and BvL varied from 25.5 to 42.02 mu g/mL for tricalcium phosphate. The three antagonistic Brevundimonas spp. showed in vitro the most inhibitory effect on mycelial growth of Fusarium redolens FRC (from 78.33 to 85.55%). Strain BvV, BvP and BvL produced also volatile metabolites which inhibited mycelial FRC growth up to 39.2%. All strains showed significant disease reduction in pot experiments. Chickpea Fusarium yellows severity caused by FRC was reduced significantly from 89.3 to 96.6% in the susceptible cultivar ILC 482 treated with antagonistic B. naejangsanensis. The maximum stimulatory effect on chickpea plants growth was observed by inoculation of strain BvV. This treatment resulted in a 7.40-26.21% increase in shoot height as compared to the control plants. It is concluded that the endophytic bacterial strains of B. naejangsanensis having different plant growth promoting (PGP) activities can be considered as beneficial microbes for sustainable agriculture. To our knowledge, this is the first report to use B. naejangsanensis strains as a new biocontrol agent against F. redolens, a new pathogen of chickpea plants causing Fusarium yellows disease in Algeria.
引用
收藏
页码:835 / 846
页数:12
相关论文
共 70 条
[1]  
Abraham WR, 1999, INT J SYST SCI
[2]  
Ali E, 2023, Journal of Plant Protection and Pathology, V0, P153, DOI [10.21608/jppp.2023.204453.1140, 10.21608/jppp.2023.204453.1140, DOI 10.21608/JPPP.2023.204453.1140]
[3]   Systematics of key phytopathogenic Fusarium species: current status and future challenges [J].
Aoki, Takayuki ;
O'Donnell, Kerry ;
Geiser, David M. .
JOURNAL OF GENERAL PLANT PATHOLOGY, 2014, 80 (03) :189-201
[4]   Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste [J].
Apun, K ;
Jong, BC ;
Salleh, MA .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2000, 46 (05) :263-267
[5]  
ASHOUR WE, 2016, BRAZ ARCH BIOL TECHN, V59, DOI DOI 10.1590/1678-4324-2016160245
[6]   Genetic diversity in Fusarium oxysporum f sp dianthi and Fusarium redolens f sp dianthi [J].
Baayen, RP ;
vanDreven, F ;
Krijger, MC ;
Waalwijk, C .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1997, 103 (05) :395-408
[7]   Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease [J].
Baayen, RP ;
O'Donnell, K ;
Bonants, PJM ;
Cigelnik, E ;
Kroon, LPNM ;
Roebroeck, EJA ;
Waalwijk, C .
PHYTOPATHOLOGY, 2000, 90 (08) :891-900
[8]   Fusarium redolens f.sp asparagi, causal agent of asparagus root rot, crown rot and spear rot [J].
Baayen, RP ;
van den Boogert, PHJF ;
Bonants, PJM ;
Poll, JTK ;
Blok, WJ ;
Waalwijk, C .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2000, 106 (09) :907-912
[9]  
Bekkar A. A., 2016, Biopesticides International, V12, P37
[10]  
Bekkar AA, 2015, THESIS U MUSTAPHA ST