A signal-off aptasensor for the determination of Acinetobacter baumannii by using methylene blue as an electrochemical probe

被引:7
作者
Abedi, Rokhsareh [1 ]
Raoof, Jahan Bakhsh [1 ]
Mohseni, Mojtaba [2 ]
Hashkavayi, Ayemeh Bagheri [3 ]
机构
[1] Univ Mazandaran, Fac Chem, Dept Analyt Chem, Electroanalyt Chem Res Lab, Babolsar, Iran
[2] Univ Mazandaran, Dept Mol & Cell Biol, Babolsar 4741695447, Iran
[3] North Carolina State Univ, Dept Elect & Comp Engn, Raleigh, NC 27606 USA
关键词
Acinetobacter baumannii; Electrochemical; Modified carbon; Screen-printed electrode; Differential pulse voltammetry; Nanocomposite; Methylene blue; GLASSY-CARBON ELECTRODE; LABEL-FREE DETECTION; SENSITIVE DETECTION; ULTRASENSITIVE DETECTION; NANOCOMPOSITE; BACTERIA; APTAMERS; DNA; IDENTIFICATION; FABRICATION;
D O I
10.1007/s00604-023-05901-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An electrochemical aptasensor has been developed to detect Acinetobacter baumannii (A. baumannii). The proposed system was developed by modifying carbon screen-printed electrodes (CSPEs) with a synthesized MWCNT@Fe3O4@SiO2-Cl nanocomposite and then binding A. baumannii-specific aptamer using covalent immobilization on the modified electrode surface and the interaction of methylene blue (MB) with Apt as an electrochemical redox indicator. As a result of the incubation of the A. baumannii bacteria as a target on the proposed aptasensor, a cathodic peak current density (J(pc)) of MB decreased due to the formation of the Apt-A. baumannii complex and MB being released from the immobilized Apt on the surface of the modified electrode. In addition to increasing the electron transfer kinetics, the nanocomposite provides a relatively stable matrix to improve the loading Apt sequence. The suggested aptasensor was demonstrated to be capable of detecting A. baumannii with a linear range of 10.0-1.0 x 10(7) colony-forming unit (CFU) mL(-1) and a detection limit of 1 CFU mL(-1) (S/N = 3) using differential pulse voltammetry (DPV) studies at a working potential of similar to 0.29 V and a scan rate of 100 mV s(-1). The outcomes revealed that the aptasensor exhibited high A. baumannii detection sensitivity, stability, reproducibility, and specificity.
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页数:11
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