Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants

被引：2

作者：

Kawakubo, Ayaka ^{[1
]}

Gallois, Jean-Luc ^{[2
]}

Nakahara, Kenji S. ^{[1
,3
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan

[2] GAFL, INRAE, F-84143 Montfavet, France

[3] Hokkaido Univ, Res Fac Agr, Sapporo, Hokkaido 0608589, Japan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2023年 / 89卷 / 01期

关键词：

iLOV; Cucumber mosaic virus; In vivo monitoring viral spread; EXPRESSION;

D O I：

10.1007/s10327-022-01100-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Tracking virus infection in plants is useful to assess plant susceptibility or resistance. The recombinant cucumber mosaic virus (CMV) expressing a 2b-fused green fluorescent protein (GFP, 25 kDa) gradually loses (within weeks) the GFP gene in plants. Here, we constructed CMV expressing a flavin-based small fluorescent protein (iLOV, 10 kDa). CMV-iLOV still retains iLOV after 28 dpi in upper leaves of inoculated Nicotiana benthamiana and allows long-term monitoring of its distribution in plants. Using CMV-iLOV, we showed that pot1, a recessive allele for resistance to potyviruses, does not confer CMV resistance in tomato.

引用

页码：47 / 52

页数：6

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