An injectable gelatin/sericin hydrogel loaded with human umbilical cord mesenchymal stem cells for the treatment of uterine injury

被引:28
作者
Chen, Lixuan [1 ,2 ]
Li, Ling [3 ]
Mo, Qinglin [4 ]
Zhang, Xiaomin [1 ,2 ]
Chen, Chaolin [4 ]
Wu, Yingnan [4 ]
Zeng, Xiaoli [5 ]
Deng, Kaixian [6 ]
Liu, Nanbo [7 ]
Zhu, Ping [7 ]
Liu, Mingxing [8 ]
Xiao, Yang [1 ,9 ]
机构
[1] Guangzhou Univ Chinese Med, Guangzhou 510006, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Jinshazhou Hosp, Guangzhou, Guangdong, Peoples R China
[3] Jiangmen Matern & Child Hlth Care Hosp, Jiangmen, Guangdong, Peoples R China
[4] Guangzhou Med Univ, Affiliated Hosp 2, Translat Med Ctr, Guangzhou, Guangdong, Peoples R China
[5] Natl Seed Cell Bank South China Tissue Engn, Guangzhou, Guangdong, Peoples R China
[6] Southern Med Univ, Peoples Hosp Shunde 1, Shunde Hosp, Dept Gynecol, Foshan, Guangdong, Peoples R China
[7] Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Guangzhou 510100, Peoples R China
[8] Guangzhou Med Univ, Affiliated Hosp 3, Dept Obstet & Gynecol, Key Lab Major Obstetr Dis Guangdong Prov, Guangzhou, Guangdong, Peoples R China
[9] Shenzhen Qianhai Shekou Pilot Free Trade Zone Hos, Shenzhen 518101, Guangdong, Peoples R China
关键词
fertility restoration; methacrylate gelatin (GelMA); methacrylate sericin (SerMA); regeneration; stem cells; uterine injury; BIOMIMETIC EXTRACELLULAR-MATRIX; GRAPHENE OXIDE; REGENERATION; SCAFFOLD; REPAIR;
D O I
10.1002/btm2.10328
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Abnormal endometrial receptivity is a major cause of the failure of embryo transplantation, which may lead to infertility, adverse pregnancy, and neonatal outcomes. While hormonal treatment has dramatically improved the fertility outcomes in women with endometriosis, a substantial unmet need persists in the treatment. In this study, methacrylate gelatin (GelMA) and methacrylate sericin (SerMA) hydrogel with human umbilical cord mesenchymal stem cells (HUMSC) encapsulation was designed for facilitating endometrial regeneration and fertility restoration through in situ injection. The presented GelMA/10%SerMA hydrogel showed appropriate swelling ratio, good mechanical properties, and degradation stability. In vitro cell experiments showed that the prepared hydrogels had excellent biocompatibility and cell encapsulation ability of HUMSC. Further in vivo experiments demonstrated that GelMA/SerMA@HUMSC hydrogel could increase the thickness of endometrium and improve the endometrial interstitial fibrosis. Moreover, regenerated endometrial tissue was more receptive to transfer embryos. Summary, we believed that GelMA/SerMA@HUMSC hydrogel will hold tremendous promise to repair or regenerate damaged endometrium.
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页数:15
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