A simplified version of rapid susceptibility testing of bacteria and yeasts using optical nanomotion detection

被引：2

作者：

Villalba, Maria I. ^{[1
,2
]}

Gligorovski, Vojislav ^{[3
]}

Rahi, Sahand J. ^{[3
]}

Willaert, Ronnie G. ^{[2
,4
]}

Kasas, Sandor ^{[1
,2
,5
]}

机构：

[1] Univ Lausanne, Ecole Polytech Fed Lausanne EPFL, Lab Biol Electron Microscopy LBEM, Lausanne, Switzerland

[2] Int Joint Res Grp VUB EPFL BioNanotechnol & Nanome, Brussels, Switzerland

[3] Ecole Polytech Fed Lausanne EPFL, Inst Phys, Lab Phys Biol Syst LPBS, Lausanne, Switzerland

[4] Vrije Univ Brussel, Res Grp Struct Biol Brussels, Alliance Res Grp VUB UGent NanoMicrobiol NAMI, Brussels, Belgium

[5] Univ Lausanne, Ctr Univ Romand Med Legale UFAM, Lausanne, Switzerland

来源：

FRONTIERS IN MICROBIOLOGY | 2024年 / 15卷

基金：

比利时弗兰德研究基金会;

关键词：

nanomotion; optical microscope; bacteria; yeast; antimicrobials; ANTIBIOTIC SUSCEPTIBILITY; MOTION;

D O I：

10.3389/fmicb.2024.1328923

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present a novel optical nanomotion-based rapid antibiotic and antifungal susceptibility test. The technique consisted of studying the effects of antibiotics or antifungals on the nanometric scale displacements of bacteria or yeasts to assess their sensitivity or resistance to drugs. The technique relies on a traditional optical microscope, a video camera, and custom-made image analysis software. It provides reliable results in a time frame of 2-4 h and can be applied to motile, non-motile, fast, and slowly growing microorganisms. Due to its extreme simplicity and low cost, the technique can be easily implemented in laboratories and medical centers in developing countries.

引用

页数：7

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