Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in Arabidopsis thaliana

被引：0

作者：

Kotlova, Ekaterina R. ^{[1
]}

Senik, Svetlana V. ^{[1
]}

Pozhvanov, Gregory A. ^{[1
,2
]}

Prokopiev, Ilya A. ^{[1
]}

Boldyrev, Ivan A. ^{[3
]}

Manzhieva, Bairta S. ^{[1
]}

Amigud, Ekaterina Ya. ^{[1
,2
]}

Puzanskiy, Roman K. ^{[1
]}

Khakulova, Anna A. ^{[4
]}

Serebryakov, Evgeny B. ^{[4
]}

机构：

[1] Russian Acad Sci, Komarov Bot Inst, St Petersburg 197022, Russia

[2] Herzen State Pedag Univ, Fac Biol, Dept Bot & Ecol, St Petersburg 191186, Russia

[3] Russian Acad Sci, Frumkin Inst Phys Chem & Electrochem, Moscow 119991, Russia

[4] St Petersburg State Univ, Chem Anal & Mat Res Core Facil Ctr, Reseach Pk, St Petersburg 199034, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

lipid uptake; plant lipids; lipidomics; phospholipids; Arabidopsis thaliana; MEMBRANE CONTACT SITES; PHYSICAL-PROPERTIES; FATTY-ACIDS; TRIACYLGLYCEROL SYNTHESIS; LIPIDS; ACCUMULATION; DIVERSITY; FLUX; PHOSPHOLIPIDS; GLYCEROLIPIDS;

D O I：

10.3390/ijms25010089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fungi and plants are not only capable of synthesizing the entire spectrum of lipids de novo but also possess a well-developed system that allows them to assimilate exogenous lipids. However, the role of structure in the ability of lipids to be absorbed and metabolized has not yet been characterized in detail. In the present work, targeted lipidomics of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), in parallel with morphological phenotyping, allowed for the identification of differences in the effects of PC molecular species introduced into the growth medium, in particular, typical bacterial saturated (14:0/14:0, 16:0/16:0), monounsaturated (16:0/18:1), and typical for fungi and plants polyunsaturated (16:0/18:2, 18:2/18:2) species, on Arabidopsis thaliana. For comparison, the influence of an artificially synthesized (1,2-di-(3-(3-hexylcyclopentyl)-propanoate)-sn-glycero-3-phosphatidylcholine, which is close in structure to archaeal lipids, was studied. The phenotype deviations stimulated by exogenous lipids included changes in the length and morphology of both the roots and leaves of seedlings. According to lipidomics data, the main trends in response to exogenous lipid exposure were an increase in the proportion of endogenic 18:1/18:1 PC and 18:1_18:2 PC molecular species and a decrease in the relative content of species with C18:3, such as 18:3/18:3 PC and/or 16:0_18:3 PC, 16:1_18:3 PE. The obtained data indicate that exogenous lipid molecules affect plant morphology not only due to their physical properties, which are manifested during incorporation into the membrane, but also due to the participation of exogenous lipid molecules in the metabolism of plant cells. The results obtained open the way to the use of PCs of different structures as cellular regulators.

引用

页数：22