Live-cell imaging and analysis of actin-mediated mitochondrial fission

被引：3

作者：

Shimura, Daisuke ^{[1
,2
]}

Shaw, Robin M. ^{[1
]}

机构：

[1] Univ Utah, Nora Eccles Harrison Cardiovasc Res & Training Ins, Salt Lake City, UT 84112 USA

[2] Univ Utah, Sch Med, Dept Surg, Salt Lake City, UT 84112 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Cell Biology; Microscopy; Molecular Biology;

D O I：

10.1016/j.xpro.2022.101958

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current approaches, such as fixed-cell imaging or single-snapshot imaging, are insufficient to capture cytoskeleton-mediated mitochondrial fission. Here, we present a protocol to capture actin-mediated mitochondrial fission using high -resolution time-lapse imaging. We describe steps starting from cell preparation and mitochondria labeling through to live-cell imaging and final analysis. This approach is also applicable for analysis of multiple cytoskeleton-mediated organelle events such as vesicle trafficking, membrane fusion, and endocytic events in live cells. For complete details on the use and execution of this protocol, please refer to Shimura et al. (2021).1

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页数：11

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