Comparison of diagnostic performance of RT-qPCR, RT-LAMP and IgM/IgG rapid tests for detection of SARS-CoV-2 among healthcare workers in Brazil

被引：2

作者：

Bernardes, Wilma Patricia de Oliveira Santos ^{[1
]}

Santos, Thais Garcia ^{[1
]}

Fernandes, Nubia Monteiro Goncalves Soares ^{[1
]}

Silva, Thais Barbara de Souza ^{[2
]}

Westin, Mateus ^{[3
]}

Simoes, Taynana Cesar ^{[4
]}

Fernandes e Silva, Eduardo ^{[4
]}

Alves, Breno Magalhaes ^{[5
]}

Molina, Israel ^{[6
]}

Melo, Mariana de Carvalho ^{[5
]}

do Monte-Neto, Rubens Lima ^{[7
]}

da Silva-Pereira, Rosiane A. ^{[1
]}

Alves, Pedro Augusto ^{[2
]}

Fonseca, Cristina Toscano ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz, Grp Pesquisa Biol & Imunol Doencas Infecciosas & P, Inst Rene Rachou, Belo Horizonte, MG, Brazil

[2] Fundacao Oswaldo Cruz, Grp Imunol Doencas Virais, Inst Rene Rachou, Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Fac Med, Belo Horizonte, MG, Brazil

[4] Fundacao Oswaldo Cruz, Nucl Estudos Saude Publ & Envelhecimento, Inst Rene Rachou, Belo Horizonte, MG, Brazil

[5] Hosp Metropolitano Doutor Celio Castro, Belo Horizonte, MG, Brazil

[6] Fundacao Oswaldo Cruz, Grp Pesquisa Triatomineos & Epidemiol Doenca Chaga, Inst Rene Rachou, Belo Horizonte, MG, Brazil

[7] Fundacao Oswaldo Cruz, Grp Pesquisa Biotecnol Aplicada Estudo Patogenos B, Inst Rene Rachou, Belo Horizonte, MG, Brazil

来源：

JOURNAL OF INFECTION AND PUBLIC HEALTH | 2023年 / 16卷 / 07期

关键词：

SARS-CoV-2; RT-LAMP; RT-qPCR; TR DPP (R) COVID-19 IgM/IgG Bio-Manguinhos; Accuracy; COVID-19;

D O I：

10.1016/j.jiph.2023.05.009

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Background: COVID-19 has become a major public health problem after the outbreak caused by SARS-CoV-2 virus. Great efforts to contain COVID-19 transmission have been applied worldwide. In this context, accurate and fast diagnosis is essential. Methods: In this prospective study, we evaluated the clinical performance of three different RNA-based molecular tests - RT-qPCR (Charite protocol), RT-qPCR (CDC (USA) protocol) and RT-LAMP - and one rapid test for detecting anti-SARS-CoV-2 IgM and IgG antibodies. Results: Our results demonstrate that RT-qPCR using the CDC (USA) protocol is the most accurate diagnostic test among those evaluated, while oro-nasopharyngeal swabs are the most appropriate biological sample. RT-LAMP was the RNA-based molecular test with lowest sensitivity while the serological test presented the lowest sensitivity among all evaluated tests, indicating that the latter test is not a good predictor of disease in the first days after symptoms onset. Additionally, we observed higher viral load in individuals who reported more than 3 symptoms at the baseline. Nevertheless, viral load had not impacted the probability of testing positive for SARS-CoV-2. Conclusion: Our data indicates that RT-qPCR using the CDC (USA) protocol in oro-nasopharyngeal swabs samples should be the method of choice to diagnosis COVID-19. (c) 2023 Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：1081 / 1088

页数：8

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