Cannabidiol protects the liver from α-Amanitin-induced apoptosis and oxidative stress through the regulation of Nrf2

被引：11

作者：

Wang, Haowei ^{[1
]}

Yang, Genmeng ^{[1
]}

Zhang, Xiaoxing ^{[1
]}

Zhang, Huijie ^{[1
]}

Liu, Yan ^{[1
]}

Wang, Chan ^{[1
]}

Miao, Lin ^{[1
]}

Li, Yi ^{[1
]}

Huang, Yizhen ^{[1
]}

Teng, Hanxin ^{[2
]}

Wang, Shangwen ^{[1
,3
]}

Cheng, Hao ^{[1
,3
]}

Zeng, Xiaofeng ^{[1
,3
]}

机构：

[1] Kunming Med Univ, Sch Forens Med, Dept Forens Med, NHC Key Lab Drug Addict Med, Kunming, Peoples R China

[2] Kunming Med Univ, Sch Basic Med Sci, Dept Pathogen Biol & Immunol, Kunming, Peoples R China

[3] Kunming Med Univ, Sch Forens Med, Dept Forens Med, NHC Key Lab Drug Addict Med, 1168 West Chunrong Rd,Yuhua Ave, Kunming 650500, Yunnan, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2023年 / 182卷

关键词：

alpha-Amanitin; Apoptosis; Cannabidiol; Hepatocytes; Nuclear factor erythroid 2-related factor 2; (Nrf2); HEPATOTOXICITY; MITOCHONDRIA; MECHANISMS; AUTOPHAGY; PATHWAY; MICE; P53;

D O I：

10.1016/j.fct.2023.114196

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

alpha-Amanitin, the primary lethal toxin of Amanita, specifically targets the liver, causing oxidative stress, hepatocyte apoptosis, and irreversible liver damage. As little as 0.1 mg/kg of alpha-amanitin can be lethal for humans, and there is currently no effective antidote for alpha-amanitin poisoning. Cannabidiol is a non-psychoactive natural compound derived from Cannabis sativa that exhibits a wide range of anti-inflammatory, antioxidant, and antiapoptotic effects. It may play a protective role in preventing liver damage induced by alpha-amanitin. To investigate the potential protective effects of cannabidiol on alpha-amanitin-induced hepatocyte apoptosis and oxidative stress, we established alpha-amanitin exposure models using C57BL/6J mice and L-02 cells in vitro. Our results showed that alpha-amanitin exposure led to oxidative stress, apoptosis, and DNA damage in both mouse hepatocytes and L-02 cells, resulting in the death of mice. We also found that cannabidiol upregulated the level of Nrf2 and antioxidant enzymes, alleviating apoptosis, and oxidative stress in mouse hepatocytes and L-02 cells and increasing the survival rate of mice. Our findings suggest that cannabidiol has hepatoprotective effects through the regulation of Nrf2 and antioxidant enzymes and may be a potential therapeutic drug for Amanita poisoning.

引用

页数：11

共 59 条

[1] Transcriptional blockade induces p53-dependent apoptosis associated with translocation of p53 to mitochondria [J].