Egg activation at fertilization in mouse eggs is caused by a series of cytosolic Ca2+ oscil-lations that are associated with an increase in ATP concentrations driven by increased mitochondrial activity. We have investigated the role of Ca2+ oscillations in these changes in ATP at fertilization by measuring the dynamics of ATP and Ca2+ in mouse eggs. An initial ATP increase started with the first Ca2+ transient at fertilization and then a second-ary increase in ATP occurred similar to 1 h later and this preceded a small and temporary increase in the frequency of Ca2+ oscillations. Other stimuli that caused Ca2+ oscillations such as PLCz1 or thimerosal, caused smaller or slower changes in ATP that failed to show the distinct secondary rise. Sperm-induced Ca2+ oscillations in the egg also triggered changes in the fluorescence of NADH which followed the pattern of Ca2+ spikes in a similar pattern to oscillations triggered by PLCz1 or thimerosal. When eggs were loaded with low concentrations of the Ca2+ chelator BAPTA, sperm triggered one small Ca2+ increase, but there were still extra phases of ATP increase that were similar to control fer-tilized eggs. Singular Ca2+ increases caused by thapsigargin were much less effective in elevating ATP levels. Together these data suggest that the secondary ATP increase at fer-tilization in mouse eggs is not caused by increases in cytosolic Ca2+. The fertilizing sperm may stimulate ATP production in eggs via both Ca2+ and by another mechanism that is independent of PLCz1 or Ca2+ oscillations.
机构:Cardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, Wales
Cox, LJ
Larman, MG
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机构:Cardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, Wales
Larman, MG
Saunders, CM
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机构:Cardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, Wales
Saunders, CM
Hashimoto, K
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机构:Cardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, Wales
Hashimoto, K
Swann, K
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机构:Cardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, Wales
Swann, K
Lai, FA
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Cardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, WalesCardiff Univ, Coll Med, Wales Heart Res Inst, Cell Signalling Lab, Cardiff CF14 4XN, S Glam, Wales
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Univ Massachusetts, Program Mol & Cellular Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Vet & Anim Sci, Paige Lab 411, Amherst, MA 01003 USA
Lee, Bora
Yoon, Sook-Young
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机构:Univ Massachusetts, Dept Vet & Anim Sci, Paige Lab 411, Amherst, MA 01003 USA
Yoon, Sook-Young
Malcuit, Chris
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机构:Univ Massachusetts, Dept Vet & Anim Sci, Paige Lab 411, Amherst, MA 01003 USA
Malcuit, Chris
Parys, Jan B.
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Katholieke Univ Leuven, Dept Mol Cell Biol, Lab Mol & Cellular Signaling, Louvain, BelgiumUniv Massachusetts, Dept Vet & Anim Sci, Paige Lab 411, Amherst, MA 01003 USA
Parys, Jan B.
Fissore, Rafael A.
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Univ Massachusetts, Dept Vet & Anim Sci, Paige Lab 411, Amherst, MA 01003 USAUniv Massachusetts, Dept Vet & Anim Sci, Paige Lab 411, Amherst, MA 01003 USA