Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+oscillations

被引:2
|
作者
Ikie-Eshalomi, Cindy [1 ]
Aliyev, Elnur [1 ]
Hoehn, Sven [1 ]
Jurkowski, Tomasz P. [1 ]
Swann, Karl [1 ]
机构
[1] Cardiff Univ, Sch Biosci, Sir Martin Evans Bldg,Museum Ave, Cardiff CF10 3AX, Wales
关键词
CA2+ OSCILLATIONS; CALCIUM OSCILLATIONS; MAMMALIAN EGGS; FERTILIZATION; ACTIVATION; OOCYTES;
D O I
10.1042/BCJ20230065
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Egg activation at fertilization in mouse eggs is caused by a series of cytosolic Ca2+ oscil-lations that are associated with an increase in ATP concentrations driven by increased mitochondrial activity. We have investigated the role of Ca2+ oscillations in these changes in ATP at fertilization by measuring the dynamics of ATP and Ca2+ in mouse eggs. An initial ATP increase started with the first Ca2+ transient at fertilization and then a second-ary increase in ATP occurred similar to 1 h later and this preceded a small and temporary increase in the frequency of Ca2+ oscillations. Other stimuli that caused Ca2+ oscillations such as PLCz1 or thimerosal, caused smaller or slower changes in ATP that failed to show the distinct secondary rise. Sperm-induced Ca2+ oscillations in the egg also triggered changes in the fluorescence of NADH which followed the pattern of Ca2+ spikes in a similar pattern to oscillations triggered by PLCz1 or thimerosal. When eggs were loaded with low concentrations of the Ca2+ chelator BAPTA, sperm triggered one small Ca2+ increase, but there were still extra phases of ATP increase that were similar to control fer-tilized eggs. Singular Ca2+ increases caused by thapsigargin were much less effective in elevating ATP levels. Together these data suggest that the secondary ATP increase at fer-tilization in mouse eggs is not caused by increases in cytosolic Ca2+. The fertilizing sperm may stimulate ATP production in eggs via both Ca2+ and by another mechanism that is independent of PLCz1 or Ca2+ oscillations.
引用
收藏
页码:2023 / 2035
页数:13
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