The Breeding of Waxy Sorghum Using Traditional Three-Line Method and Marker-Assisted Selection

被引:0
|
作者
Wu, Yong-Pei [1 ]
Chang, Yu-Chi [1 ]
Kuo, Su-Chen [1 ]
Liao, Dah-Jing [1 ]
Shen, Ting-Yu [1 ]
Kuo, Hsin-, I [1 ,2 ]
Wang, Sheng-Wen [2 ]
Tseng, Yu-Chien [2 ]
机构
[1] Minist Agr, Taiwan Agr Res Inst, Chiayi Agr Expt Branch, Chiayi 60015, Taiwan
[2] Natl Chiayi Univ, Dept Agron, Chiayi 60004, Taiwan
来源
AGRICULTURE-BASEL | 2023年 / 13卷 / 11期
关键词
backcrossing; foreground selection; granule-bound starch synthase I; hybrid; MOLECULAR CHARACTERIZATION; ALLELES; STARCH; GENE; IDENTIFICATION; INSERTION; LOCUS; WHEAT;
D O I
10.3390/agriculture13112054
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Sorghum (Sorghum bicolor) exhibits drought resistance and environmental adaptability, making it a crucial cereal crop for semi-arid regions. It has a wide range of uses, including as food, feed, brooms, alcohol production, and bioethanol. In particular, Taiwan imports nearly 50,000 tons of sorghum annually, primarily for the production of sorghum liquor. However, the ideal raw material for high-quality sorghum liquor is waxy sorghum, and not all sorghum varieties imported or promoted in Taiwan are of this waxy type. Consequently, there is a shortage of sufficient waxy sorghum raw materials to meet the demands of the Taiwan market. The occurrence of waxy sorghum (wx) is caused by the mutation of granule-bound starch synthase I (GBBS I), and there are currently several known types of mutants, carrying different wxa, wxb, and wxc waxy alleles. Among them, wxc is a novel mutation type, and in native sorghum in Taiwan, individuals with the waxy allele wxc have been found. The three-line method is a commonly used breeding strategy, which simplifies the process of emasculation to obtain hybrid F1 offspring. In this study, imported sorghum variety Liangnuo No.1 (with male sterility), native glutinous sorghum variety SB6 from Taiwan (carrying the wxc waxy allele), and sorghum reference genome variety BTx623 were used as research materials. The goal was to use the three-line method to produce waxy sorghums, including the male sterile line (A-line), male sterile maintenance line (B-line), and fertility-restoring line (R-line). The breeding results showed that by using backcross breeding, molecular-assisted selection, and traditional field selection methods, high-quality three-line materials (A-, B-, R-lines, named CNA1, CNB1 CNR1, respectively) and F1 hybrid (CNH1) with favorable agronomic traits and yield quality were successfully obtained.
引用
收藏
页数:14
相关论文
共 50 条
  • [31] Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench)
    Jianming Gao
    Buxian Xia
    Feng Luo
    Shoujun Sun
    Zhongyou Pei
    Zhi Gui
    Qinghua Yuan
    Xianglin Li
    Euphytica, 2013, 193 : 383 - 390
  • [32] Marker-assisted selection for transfer of QTLs to a promising line for drought tolerance in wheat (Triticum aestivum L.)
    Sunilkumar, V. P.
    Krishna, Hari
    Devate, Narayana Bhat
    Manjunath, Karthik Kumar
    Chauhan, Divya
    Singh, Shweta
    Sinha, Nivedita
    Singh, Jang Bahadur
    Prakasha, T. L.
    Pal, Dharam
    Sivasamy, M.
    Jain, Neelu
    Singh, Gyanendra Pratap
    Singh, Pradeep Kumar
    FRONTIERS IN PLANT SCIENCE, 2023, 14
  • [33] Molecular markers for the identification of resistance genes and marker-assisted selection in breeding wheat for leaf rust resistance
    Vida, Gyula
    Gal, Mariann
    Uhrin, Andrea
    Veisz, Otto
    Syed, Naeem Hasan
    Flavell, Andrew J.
    Wang, Zhulin
    Bedo, Zoltan
    EUPHYTICA, 2009, 170 (1-2) : 67 - 76
  • [34] Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Rygene) in a Tetraploid Potato Breeding Program
    Ottoman, Ryon J.
    Hane, Dan C.
    Brown, Charles R.
    Yilma, Solomon
    James, Steven R.
    Mosley, Alvin R.
    Crosslin, James M.
    Vales, M. Isabel
    AMERICAN JOURNAL OF POTATO RESEARCH, 2009, 86 (04) : 304 - 314
  • [35] A Comparative Analysis of Conventional and Marker-Assisted Selection Methods in Breeding Maize Streak Virus Resistance in Maize
    Abalo, Grace
    Tongoona, Pangirayi
    Derera, John
    Edema, Richard
    CROP SCIENCE, 2009, 49 (02) : 509 - 520
  • [36] Marker-Assisted Selection for Early Maturing E Loci in Soybean Yielded Prospective Breeding Lines for High Latitudes of Northern Kazakhstan
    Yerzhebayeva, Raushan
    Didorenko, Svetlana
    Amangeldiyeva, Aigul
    Daniyarova, Aliya
    Mazkirat, Shynar
    Zinchenko, Alyona
    Shavrukov, Yuri
    BIOMOLECULES, 2023, 13 (07)
  • [37] Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding
    He, Jiangfeng
    Zhao, Xiaoqing
    Laroche, Andre
    Lu, Zhen-Xiang
    Liu, HongKui
    Li, Ziqin
    FRONTIERS IN PLANT SCIENCE, 2014, 5
  • [38] Molecular markers for the identification of resistance genes and marker-assisted selection in breeding wheat for leaf rust resistance
    Gyula Vida
    Mariann Gál
    Andrea Uhrin
    Ottó Veisz
    Naeem Hasan Syed
    Andrew J. Flavell
    Zhulin Wang
    Zoltán Bedő
    Euphytica, 2009, 170 : 67 - 76
  • [39] Improvement of three commercial spring wheat varieties for powdery mildew resistance by marker-assisted selection
    Ye, Xingguo
    Zhang, Shuangxi
    Li, Shijin
    Wang, Jing
    Chen, Haiqiang
    Wang, Ke
    Lin, Zhishan
    Wei, Yiqin
    Du, Lipu
    Yan, Yueming
    CROP PROTECTION, 2019, 125
  • [40] Development of SNP markers for marker-assisted breeding in Chinese cabbage using Fluidigm genotyping assays
    Su Ryun Choi
    Sang Heon Oh
    Vignesh Dhandapani
    Chang Soon Jang
    Chun-Hee Ahn
    Jana Jeevan Rameneni
    Hyuna Kim
    Inbae Jeon
    Yong Pyo Lim
    Horticulture, Environment, and Biotechnology, 2020, 61 : 327 - 338