Incorporation of Cell-Adhesive Proteins in 3D-Printed Lipoic Acid-Maleic Acid-Poly(Propylene Glycol)-Based Tough Gel Ink for Cell-Supportive Microenvironment

被引:4
作者
Tran, Hao Nguyen [1 ]
Kim, In Gul [2 ]
Kim, Jong Heon [3 ]
Bhattacharyya, Amitava [1 ,3 ]
Chung, Eun-Jae [2 ]
Noh, Insup [1 ,3 ]
机构
[1] Seoul Natl Univ Sci & Technol, Dept Chem & Biomol Engn, Seoul 01811, South Korea
[2] Seoul Natl Univ Hosp, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Seoul 03080, South Korea
[3] Seoul Natl Univ Sci & Technol, Convergence Inst Biomed Engn & Biomat, Seoul 01811, South Korea
关键词
3D printing; cell-adhesive protein; hydrogel ink; scaffold; TERPOLYMERIC HYDROGEL; GELATIN METHACRYLOYL; 3D; CONSTRUCTS; SCAFFOLD; SURFACE;
D O I
10.1002/mabi.202300316
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In extrusion-based 3D printing, the use of synthetic polymeric hydrogels can facilitate fabrication of cellularized and implanted scaffolds with sufficient mechanical properties to maintain the structural integrity and physical stress within the in vivo conditions. However, synthetic hydrogels face challenges due to their poor properties of cellular adhesion, bioactivity, and biofunctionality. New compositions of hydrogel inks have been designed to address this limitation. A viscous poly(maleate-propylene oxide)-lipoate-poly(ethylene oxide) (MPLE) hydrogel is recently developed that shows high-resolution printability, drug-controlled release, excellent mechanical properties with adhesiveness, and biocompatibility. In this study, the authors demonstrate that the incorporation of cell-adhesive proteins like gelatin and albumin within the MPLE gel allows printing of biologically functional 3D scaffolds with rapid cell spreading (within 7 days) and high cell proliferation (twofold increase) as compared with MPLE gel only. Addition of proteins (10% w/v) supports the formation of interconnected cell clusters (& AP;1.6-fold increase in cell areas after 7-day) and spreading of cells in the printed scaffolds without additional growth factors. In in vivo studies, the protein-loaded scaffolds showed excellent biocompatibility and increased angiogenesis without inflammatory response after 4-week implantation in mice, thus demonstrating the promise to contribute to the printable tough hydrogel inks for tissue engineering. Cell-adhesive proteins like gelatin and albumin within the poly(maleate-propylene oxide)-lipoate-poly(ethylene oxide) gel allows printing of biofunctional 3D scaffolds with high cell proliferation compared to pure gel. The addition of proteins supports the formation of interconnected cell clusters and spreading of cells in the printed scaffolds without additional growth factors. The protein-loaded scaffolds showed excellent biocompatibility and increased angiogenesis without inflammatory response in mice.image
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页数:14
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