Incorporation of Cell-Adhesive Proteins in 3D-Printed Lipoic Acid-Maleic Acid-Poly(Propylene Glycol)-Based Tough Gel Ink for Cell-Supportive Microenvironment

被引:4
作者
Tran, Hao Nguyen [1 ]
Kim, In Gul [2 ]
Kim, Jong Heon [3 ]
Bhattacharyya, Amitava [1 ,3 ]
Chung, Eun-Jae [2 ]
Noh, Insup [1 ,3 ]
机构
[1] Seoul Natl Univ Sci & Technol, Dept Chem & Biomol Engn, Seoul 01811, South Korea
[2] Seoul Natl Univ Hosp, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Seoul 03080, South Korea
[3] Seoul Natl Univ Sci & Technol, Convergence Inst Biomed Engn & Biomat, Seoul 01811, South Korea
关键词
3D printing; cell-adhesive protein; hydrogel ink; scaffold; TERPOLYMERIC HYDROGEL; GELATIN METHACRYLOYL; 3D; CONSTRUCTS; SCAFFOLD; SURFACE;
D O I
10.1002/mabi.202300316
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In extrusion-based 3D printing, the use of synthetic polymeric hydrogels can facilitate fabrication of cellularized and implanted scaffolds with sufficient mechanical properties to maintain the structural integrity and physical stress within the in vivo conditions. However, synthetic hydrogels face challenges due to their poor properties of cellular adhesion, bioactivity, and biofunctionality. New compositions of hydrogel inks have been designed to address this limitation. A viscous poly(maleate-propylene oxide)-lipoate-poly(ethylene oxide) (MPLE) hydrogel is recently developed that shows high-resolution printability, drug-controlled release, excellent mechanical properties with adhesiveness, and biocompatibility. In this study, the authors demonstrate that the incorporation of cell-adhesive proteins like gelatin and albumin within the MPLE gel allows printing of biologically functional 3D scaffolds with rapid cell spreading (within 7 days) and high cell proliferation (twofold increase) as compared with MPLE gel only. Addition of proteins (10% w/v) supports the formation of interconnected cell clusters (& AP;1.6-fold increase in cell areas after 7-day) and spreading of cells in the printed scaffolds without additional growth factors. In in vivo studies, the protein-loaded scaffolds showed excellent biocompatibility and increased angiogenesis without inflammatory response after 4-week implantation in mice, thus demonstrating the promise to contribute to the printable tough hydrogel inks for tissue engineering. Cell-adhesive proteins like gelatin and albumin within the poly(maleate-propylene oxide)-lipoate-poly(ethylene oxide) gel allows printing of biofunctional 3D scaffolds with high cell proliferation compared to pure gel. The addition of proteins supports the formation of interconnected cell clusters and spreading of cells in the printed scaffolds without additional growth factors. The protein-loaded scaffolds showed excellent biocompatibility and increased angiogenesis without inflammatory response in mice.image
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页数:14
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共 57 条
[11]   Characterizations of hyaluronate-based terpolymeric hydrogel synthesized via free radical polymerization mechanism for biomedical applications [J].
Das, Dipankar ;
Thi Thu Hien Pham ;
Noh, Insup .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2018, 170 :64-75
[12]   Bioprintable, cell-laden silk fibroin-gelatin hydrogel supporting multilineage differentiation of stem cells for fabrication of three-dimensional tissue constructs [J].
Das, Sanskrita ;
Pati, Falguni ;
Choi, Yeong-Jin ;
Rijal, Girdhari ;
Shim, Jin-Hyung ;
Kim, Sung Won ;
Ray, Alok R. ;
Cho, Dong-Woo ;
Ghosh, Sourabh .
ACTA BIOMATERIALIA, 2015, 11 :233-246
[13]   Caffeine-catalyzed gels [J].
DiCiccio, Angela M. ;
Lee, Young-Ah Lucy ;
Glettig, Dean L. ;
Walton, Elizabeth S. E. ;
de la Serna, Eva L. ;
Montgomery, Veronica A. ;
Grant, Tyler M. ;
Langer, Robert ;
Traverso, Giovanni .
BIOMATERIALS, 2018, 170 :127-135
[14]   Gelatin methacryloyl hydrogel as an injectable scaffold with multi-therapeutic effects to promote antimicrobial disinfection and angiogenesis for regenerative endodontics [J].
Dubey, Nileshkumar ;
Ribeiro, Juliana S. ;
Zhang, Zhaocheng ;
Xu, Jinping ;
Ferreira, Jessica A. ;
Qu, Liu ;
Mei, Ling ;
Fenno, J. Christopher ;
Schwendeman, Anna ;
Schwendeman, Steven P. ;
Nor, Jacques E. ;
Bottino, Marco C. .
JOURNAL OF MATERIALS CHEMISTRY B, 2023, 11 (17) :3823-3835
[15]   Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues [J].
Freeman, Fiona E. ;
Kelly, Daniel J. .
SCIENTIFIC REPORTS, 2017, 7
[16]   Recent trends in bioinks for 3D printing [J].
Gopinathan J. ;
Noh I. .
Biomaterials Research, 22 (1)
[17]   Synthesis of bovine serum albumin-gelatin composite adhesive hydrogels by physical crosslinking [J].
Guo, Cuiping ;
Zeng, Zhiwen ;
Yu, Shan ;
Huang, Jun ;
Geng, Zhijie ;
Pei, Dating ;
Lu, Daohuan .
JOURNAL OF POLYMER RESEARCH, 2022, 29 (07)
[18]   Mussel-Inspired Tissue-Adhesive Hydrogel Based on the Polydopamine-Chondroitin Sulfate Complex for Growth-Factor-Free Cartilage Regeneration [J].
Han, Lu ;
Wang, Menghao ;
Li, Pengfei ;
Gan, Donglin ;
Yan, Liwei ;
Xu, Jielong ;
Wang, Kefeng ;
Fang, Liming ;
Chan, Chun Wai ;
Zhang, Hongping ;
Yuan, Huipin ;
Lu, Xiong .
ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (33) :28015-28026
[19]   Double-Network Polyurethane-Gelatin Hydrogel with Tunable Modulus for High-Resolution 3D Bioprinting [J].
Hsieh, Cheng-Tien ;
Hsu, Shan-hui .
ACS APPLIED MATERIALS & INTERFACES, 2019, 11 (36) :32746-32757
[20]   A synthetic elastomer based on acrylated polypropylene glycol triol with tunable modulus for tissue engineering applications [J].
Hudson, James E. ;
Frith, Jessica E. ;
Donose, Bogdan C. ;
Rondeau, Elisabeth ;
Mills, Richard J. ;
Wolvetang, Ernst J. ;
Brooke, Gary P. ;
Cooper-White, Justin J. .
BIOMATERIALS, 2010, 31 (31) :7937-7947