Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells

被引：5
作者：
Geng, Lihua [1 ,2 ,3 ,4 ]
Zhang, Quanbin [1 ,2 ,3 ,4 ]
Li, Qiong [5 ]
Zhang, Qian [4 ,6 ]
Wang, Cong [7 ]
Song, Ni [7 ]
Xin, Wenyu [8 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
[2] Inst Oceanol, Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
[3] Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
[4] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
[5] Shandong Marine Forecast & Hazard Mitigat Serv, Qingdao, Peoples R China
[6] Chinese Acad Sci, Inst Oceanol, Publ Technol Serv Ctr, Qingdao, Peoples R China
[7] Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China
[8] Binzhou Med Univ, Sch Pharm, Key Lab Prescript Effect & Clin Evaluat State Adm, Yantai, Shandong, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2024年 / 332卷
基金：
中国国家自然科学基金;
关键词：
Silvetia siliquosa; Fucoidan; Polysaccharide; Immunomodulatory activity; Sulfate groups; SACCHARINA-JAPONICA; BROWN SEAWEED; STRUCTURAL CHARACTERISTICS; SULFATED POLYSACCHARIDES; EXTRACTION; HETEROPOLYSACCHARIDE; FUCOSTEROL; ALGAE;
D O I：
10.1016/j.carbpol.2024.121883
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Silvetia siliquosa, the only species of the family Fucaceae in China, is used as a medicine food homology. Fucoidan from S. siliquosa was extracted by hot water twice thoroughly (13 % of total yield), and a purified fucoidan SSF with a molecular weight of 93 kD was obtained. Chemical composition analysis demonstrated that SSF was primarily composed of sulfate (21.68 wt%) and fucose (84 % of all neutral monosaccharides). IR, methylation analysis, NMR and ESI-MS results indicated SSF had the backbone of mainly (1 -* 3)-alpha-L-fucopyranose and minor (1 -* 4)-alpha-L-fucopyranose, with little 1,3 and 1,4 branched beta-D-Xylp and beta-D-Galp. The in vitro immunomodulatory test on RAW 264.7 cells showed that SSF could up-regulate the expression of immune related factors and proteins in a concentration-dependent manner, but the immunomodulatory effect disappeared from desulfated SSF. This research indicated that highly sulfated fucan possessed immunomodulatory effect and the importance of sulfate groups in the activity of SSF.
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页数：13
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共 49 条
  • [1] Sequential ultrasonic/microwave assisted extraction of fucoidan from Nizamudinia zanardinii and evaluation of its biological activities
    Alboofetileh, M.
    Rezaei, M.
    Tabarsa, M.
    Cravotto, G.
    [J]. IRANIAN JOURNAL OF FISHERIES SCIENCES, 2023, 22 (01): : 84 - 98
  • [2] Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation
    Ale, Marcel Tutor
    Meyer, Anne S.
    [J]. RSC ADVANCES, 2013, 3 (22): : 8131 - 8141
  • [3] Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry
    Anastyuk, Stanislav D.
    Shevchenko, Natalia M.
    Nazarenko, Eugene L.
    Imbs, Tatyana I.
    Gorbach, Vladimir I.
    Dmitrenok, Pavel S.
    Zvyagintseva, Tatyana N.
    [J]. CARBOHYDRATE RESEARCH, 2010, 345 (15) : 2206 - 2212
  • [4] Immunomodulatory and Anti-Inflammatory Effects of Fucoidan: A Review
    Apostolova, Elisaveta
    Lukova, Paolina
    Baldzhieva, Alexandra
    Katsarov, Plamen
    Nikolova, Mariana
    Iliev, Ilia
    Peychev, Lyudmil
    Trica, Bogdan
    Oancea, Florin
    Delattre, Cedric
    Kokova, Vesela
    [J]. POLYMERS, 2020, 12 (10) : 1 - 25
  • [5] Bilan M. I., 2005, Biochemistry, V70
  • [6] Sulfated polysaccharides of the Vietnamese brown alga Sargassum aquifolium (Fucales, Sargassaceae)
    Bilan, Maria I.
    Ustyuzhanina, Nadezhda E.
    Shashkov, Alexander S.
    Thi Thu Thuy Thanh
    Minh Ly Bui
    Thi Thanh Van Tran
    Van Nguyen Bui
    Usov, Anatolii I.
    [J]. CARBOHYDRATE RESEARCH, 2017, 449 : 23 - 31
  • [7] Further studies on the composition and structure of a fucoidan preparation from the brown alga Saccharina latissima
    Bilan, Maria I.
    Grachev, Alexey A.
    Shashkov, Alexander S.
    Kelly, Maeve
    Sanderson, Craig J.
    Nifantiev, Nikolay E.
    Usov, Anatolii I.
    [J]. CARBOHYDRATE RESEARCH, 2010, 345 (14) : 2038 - 2047
  • [8] Structure of a fucoidan from the brown seaweed Fucus serratus L.
    Bilan, MI
    Grachev, AA
    Shashkov, AS
    Nifantiev, NE
    Usov, AI
    [J]. CARBOHYDRATE RESEARCH, 2006, 341 (02) : 238 - 245
  • [9] A MODIFIED URONIC ACID CARBAZOLE REACTION
    BITTER, T
    MUIR, HM
    [J]. ANALYTICAL BIOCHEMISTRY, 1962, 4 (04) : 330 - &
  • [10] A review about brown algal cell walls and fucose-containing sulfated polysaccharides: Cell wall context, biomedical properties and key research challenges
    Deniaud-Bouet, Estelle
    Hardouin, Kevin
    Potin, Philippe
    Kloareg, Bernard
    Herve, Cecile
    [J]. CARBOHYDRATE POLYMERS, 2017, 175 : 395 - 408
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