The Effect of Far-Red Light and Nutrient Level on the Growth and Secondary Metabolites of the In Vitro Culture of Prunella vulgaris

被引:3
作者
Chen, Cai-Lin [1 ]
Yang, Jing-Ping [2 ]
Huang, Wen-Dar [3 ]
Chen, Chang-Chang [2 ]
机构
[1] Yunlin Cty Govt, Agr Dept, Touliu 515, Taiwan
[2] Natl Res Inst Chinese Med, Taipei 112, Taiwan
[3] Natl Taiwan Univ, Dept Agron, Taipei 101, Taiwan
来源
AGRONOMY-BASEL | 2023年 / 13卷 / 09期
关键词
Prunella vulgaris; far-red light; nutrient levels; plantlet growth; photosynthetic pigments; secondary metabolites; antioxidant activity; ROSMARINIC ACID; NITROGEN-FERTILIZATION; ANTIOXIDANT ACTIVITY; PHENOLIC-ACIDS; PHOTOSYNTHESIS; GENES; ACCUMULATION; RESPONSES; CAPACITY; EXTRACTS;
D O I
10.3390/agronomy13092250
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Prunella vulgaris, a medicinal plant with antioxidant capacity, was investigated for its response to varying intensities of far-red light and nutrient levels. Plantlets were cultured for 30 d under low far-red light (LFR) or high far-red light (HFR) conditions and different nutrient levels (full, half, and quarter). HFR reduced leaf and branch number, dry weight, and accumulation of chlorophylls (Chl) and carotenoids (Car), while increasing plant height. Lower nutrient levels increased plant height and leaf number, but decreased branch number, Chl, and Car. HFR significantly increased total phenolic content (TPC), rutin, and rosmarinic acid levels, while total flavonoid content decreased. As nutrient levels decreased, TPC and rosmarinic acid declined. HFR induced significant DPPH scavenging activity, while reducing power increased with higher far-red light and nutrient levels. The ferrous ion chelating effect under LFR reduced with lower nutrient levels. There were strong correlations among TPC, rosmarinic acid, DPPH scavenging activity, and reducing power. In conclusion, HFR inhibited plantlet growth but enhanced secondary metabolite accumulation and antioxidant capacity. Different nutrient levels stimulated diverse growth responses, while elevated nutrient levels promoted secondary metabolite production. This study demonstrated the responses of growth, secondary metabolite accumulation, and antioxidant activity in the in vitro cultured P. vulgaris to supplemental far-red light and various nutrient levels.
引用
收藏
页数:15
相关论文
共 71 条
[31]   Potassium in plants: Growth regulation, signaling, and environmental stress tolerance [J].
Johnson, Riya ;
Vishwakarma, Kanchan ;
Hossen, Md. Shahadat ;
Kumar, Vinod ;
Shackira, A. M. ;
Puthur, Jos T. ;
Abdi, Gholamreza ;
Sarraf, Mohammad ;
Hasanuzzaman, Mirza .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2022, 172 :56-69
[32]   Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from Prunella vulgaris [J].
Kim, Yeon Bok ;
Shin, You Jin ;
Pham Anh Tuan ;
Li, Xiaohua ;
Park, Yunji ;
Park, Nam-il ;
Park, Sang Un .
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2014, 37 (07) :1221-1227
[33]   The influence of different wavelengths of LED light on the production of glucosinolates and phenolic compounds and the antioxidant potential in in vitro cultures of Nasturtium officinale (watercress) [J].
Klimek-Szczykutowicz, Marta ;
Prokopiuk, Barbara ;
Dziurka, Kinga ;
Pawlowska, Bozena ;
Ekiert, Halina ;
Szopa, Agnieszka .
PLANT CELL TISSUE AND ORGAN CULTURE, 2022, 149 (1-2) :113-122
[34]   Phenolics and betacyanins in red beetroot (Beta vulgaris) root:: Distribution and effect of cold storage on the content of total phenolics and three individual compounds [J].
Kujala, TS ;
Loponen, JM ;
Klika, KD ;
Pihlaja, K .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (11) :5338-5342
[35]   Supplemental Far-Red Light Stimulates Lettuce Growth: Disentangling Morphological and Physiological Effects [J].
Legendre, Reeve ;
van Iersel, Marc W. .
PLANTS-BASEL, 2021, 10 (01) :1-21
[36]   ACTION SPECTRUM, ABSORPTANCE AND QUANTUM YIELD OF PHOTOSYNTHESIS IN CROP PLANTS [J].
MCCREE, KJ .
AGRICULTURAL METEOROLOGY, 1972, 9 (3-4) :191-&
[37]  
Medrado HH, 2017, J BRAZIL CHEM SOC, V28, P505
[38]   Acclimation of photosynthesis to irradiance and spectral quality in British plant species: Chlorophyll content, photosynthetic capacity and habitat preference [J].
Murchie, EH ;
Horton, P .
PLANT CELL AND ENVIRONMENT, 1997, 20 (04) :438-448
[39]   Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of basil (Ocimum basilicum L.) [J].
Nguyen, Phuong M. ;
Niemeyer, Emily D. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (18) :8685-8691
[40]   Prunella vulgaris L. - A Review of its Ethnopharmacology, Phytochemistry, Quality Control and Pharmacological Effects [J].
Pan, Junying ;
Wang, Haoyu ;
Chen, Yinghua .
FRONTIERS IN PHARMACOLOGY, 2022, 13