Rapid identification of chemical constituents in Hugan tablets by ultra-performance liquid chromatography-quadrupole-exactive orbitrap mass spectrometry

被引：3

作者：

Wang, Xiaowen ^{[1
]}

Liu, Huiru ^{[1
]}

Shu, Lexin ^{[1
]}

Yao, Yaqi ^{[1
]}

Xu, Yanyan ^{[1
]}

Wei, Jinxia ^{[1
]}

Li, Yubo ^{[1
,2
]}

机构：

[1] Tianjin Univ Tradit Chinese Med, Sch Chinese Mat Med, Tianjin, Peoples R China

[2] Tianjin Univ Tradit Chinese Med, 10 Poyang Lake Rd, West Zone,Tuanbo New City, Tianjin 301617, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2023年 / 46卷 / 19期

关键词：

Chinese medicine preparations; component analysis; Hugan Tablet; mass spectrometry; ultra-performance liquid chromatography; SCHISANDRA-CHINENSIS; ANTIOXIDANT; EXTRACT; MS/MS; METABOLITES; GERMINATION; ACIDS; L;

D O I：

10.1002/jssc.202300302

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hugan tablet is a Chinese medicine preparation. It is composed of Bupleuri Radix, Artemisiae Scopariae Herba, Isatidis Radix, Schisandrae Chinensis Fructus, Suis Fellis Pulvis, and Vigna radiata L. It has the effects of dispersing stagnated liver qi, strengthening the spleen and eliminating food to be used for the treatment of chronic hepatitis and early cirrhosis. However, the chemical composition of Hugan tablet is complex and not fully understood, which hampers the research in pharmacology. In this study, a reliable method for the rapid analysis and identification of the chemical components in Hugan tablet by their characteristic fragments and neutral losses using ultra-performance liquid chromatography-quadrupole-exactive orbitrap mass spectrometry was developed. A total of 144 chemical components were tentatively identified, including 57 organic acids, 19 flavonoids, 23 alkaloids, 18 lignans, 7 saponins, and 20 others. These components may be the active ingredients of Hugan tablet. The established method can systematically and rapidly analyze the chemical components in Hugan tablet, which provides a basis for the pharmacodynamic substance study and is meaningful for the quality control of Hugan tablet.

引用

页数：11

共 57 条

[1]

Bala M, 2015, NAT PROD COMMUN, V10, P43

[2] Evaluation of the estrogenic effects of legume extracts containing phytoestrogens [J].

Boué, SM ;

Wiese, TE ;

Nehls, S ;

Burow, ME ;

Elliott, S ;

Carter-Wientjes, CH ;

Shih, BY ;

McLachlan, JA ;

Cleveland, TE .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (08) :2193-2199

[3] Systematic characterization of chemical constituents in Mahuang decoction by UHPLC tandem linear ion trap-Orbitrap mass spectrometry coupled with feature-based molecular networking [J].

Chen, Ling ;

Yao, Changliang ;

Li, Jiayuan ;

Wang, Jing ;

Yao, Shuai ;

Shen, Shijie ;

Yang, Lin ;

Zhang, Jianqing ;

Wei, Wenlong ;

Bi, Qirui ;

Guo, De-an .

JOURNAL OF SEPARATION SCIENCE, 2021, 44 (14) :2717-2727

[4] Quality Evaluation of Wild and Cultivated Schisandrae Chinensis Fructus Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis [J].

Chen, Shuyu ;

Shi, Jingjing ;

Zou, Lisi ;

Liu, Xunhong ;

Tang, Renmao ;

Ma, Jimei ;

Wang, Chengcheng ;

Tan, Mengxia ;

Chen, Jiali .

MOLECULES, 2019, 24 (07)

[5] Quality Evaluation of Artemisia capillaris Thunb. Based on Qualitative Analysis of the HPLC Fingerprint and UFLC-Q-TOF-MS/MS Combined with Quantitative Analysis of Multicomponents [J].

Dai, Ying ;

Dou, Zhihua ;

Zhou, Rongrong ;

Luo, Lin ;

Bian, Li ;

Chen, Yufeng ;

Tao, Jinhua ;

Chen, Zhixian .

JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, 2021, 2021

[6] Evolution of Orbitrap Mass Spectrometry Instrumentation [J].

Eliuk, Shannon ;

Makarov, Alexander .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 8, 2015, 8 :61-80

[7] Qualitative and Quantitative Analysis of 24 Components in Jinlianhua Decoction by UPLC-MS/MS [J].

Fang, Mingyue ;

Liu, Shuangyue ;

Wang, Qingqing ;

Gu, Xuan ;

Ding, Pengmin ;

Wang, Weihua ;

Ding, Yi ;

Liu, Junxiu ;

Wang, Rufeng .

CHROMATOGRAPHIA, 2019, 82 (12) :1801-1825

[8] Huganpian, a traditional chinese medicine, inhibits liver cancer growth in vitro and in vivo by inducing autophagy and cell cycle arrest [J].

Gao, Xue ;

Wang, Yuyang ;

Li, Yuxin ;

Wang, Yansong ;

Yan, Miaomiao ;

Sun, Hongliu ;

Chen Shayan ;

Xiaohong Pan .

BIOMEDICINE & PHARMACOTHERAPY, 2019, 120

[9]

Gu LY, 2015, J BUON, V20, P1341

[10] Development of Highly Sensitive Method for Sugar Determination in Herbal Medicine; Application of Monosaccharides and Oligosaccharides in Japanese Angelica Root and Rehmannia Root [J].

Han, Xu ;

Ando, Hirokazu ;

Kudo, Yoshitomi ;

Sasaki, Yohei .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2022, 70 (11) :796-804

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