Synthesis of Pt nanoparticles with gelatin-assisted green route to improve sensitization of cancer cells to X-Ray irradiation

被引：8

作者：

Charmi, Jalil ^{[3
]}

Seidi, Farzad ^{[1
,2
,8
]}

Amereh, Mahdi ^{[3
]}

Ghaffarlou, Mohammadreza ^{[4
]}

Salehiabar, Marziyeh ^{[3
]}

Yousefnejad, Shima ^{[3
]}

Barsbay, Murat ^{[4
,5
]}

Sharafi, Ali ^{[3
]}

Javani, Siamak ^{[6
,7
,8
]}

Nosrati, Hamed ^{[3
,8
]}

机构：

[1] Nanjing Forestry Univ, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Nanjing 210037, Peoples R China

[2] Nanjing Forestry Univ, Innovat Ctr Forest Chem & Mat, Nanjing 210037, Peoples R China

[3] Zanjan Univ Med Sci, Zanjan Pharmaceut Biotechnol Res Ctr, Zanjan, Iran

[4] Hacettepe Univ, Dept Chem, TR-06800 Ankara, Turkiye

[5] Hacettepe Univ, Inst Sci, Polymer Sci & Technol Div, TR-06800 Ankara, Turkiye

[6] Golestan Univ Med Sci, Med Cellular & Mol Res Ctr, Gorgan, Iran

[7] Golestan Univ Med Sci, Sch Adv Technol Med, Gorgan, Iran

[8] Nanjing Forestry Univ, Nanjing 210037, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2023年 / 643卷

关键词：

Platinum NPs; Radiosensitizer; High-Z element; Reducing agent; Gelatin; GOLD NANOPARTICLES; ENHANCE RADIOSENSITIZATION; RADIATION-THERAPY;

D O I：

10.1016/j.ijpharm.2023.123148

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

This study aimed to develop a novel radiosensitizer consisting of platinum nanoparticles (Pt NPs) as a highatomic-number element in order to maximize the generation of ROS under ionizing radiation at the tumor site. Pt NPs were produced via a green and facile method in the presence of gelatin (Gel) as both reducing and stabilizing agent. After determining the physical structure and chemical composition of Pt@Gel NPs by STEM, FeSEM, EDS, DLS, XRD and FTIR, in vitro cytotoxicity on human umbilical vein endothelial cells (HUVEC) and breast cancer cell line (4T1) was evaluated by MTT assay. Finally, ROS generation assay, calcein AM/PI staining assay and clonogenic test were performed on 4T1 cells under X-Ray irradiation to evaluate the radioenhancment efficiency of Pt@Gel. The prepared NPs exhibited spherical and uniform shapes and narrowly distributed sizes in addition to an acceptable radiosensitization capability. The nanosystem provided higher levels of intracellular ROS in malignant cells and enhanced cancer cell death rate under X-Ray irradiation. Overall, the findings suggested that Pt@Gel could be a safe and effective alternative to existing radiosensitizers and potentially be employed for the treatment of breast cancer.

引用

页数：7

共 38 条

[1] Main Approaches to Enhance Radiosensitization in Cancer Cells by Nanoparticles: A Systematic Review [J].