Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69

被引：54

作者：

Li, Yinghui ^{[1
,2
]}

Wei, Zhen-Zhen ^{[1
,2
,3
]}

Sela, Hanan ^{[1
,2
]}

Govta, Liubov ^{[1
,2
]}

Klymiuk, Valentyna ^{[1
,2
,4
,5
]}

Roychowdhury, Rajib ^{[1
,2
]}

Chawla, Harmeet Singh ^{[4
,5
,10
]}

Ens, Jennifer ^{[4
,5
]}

Wiebe, Krystalee ^{[4
,5
]}

Bocharova, Valeria ^{[1
,2
]}

Ben-David, Roi ^{[1
,2
,6
]}

Pawar, Prerna B. ^{[1
,2
]}

Zhang, Yuqi ^{[7
]}

Jaiwar, Samidha ^{[1
,2
]}

Molnar, Istvan ^{[8
,11
]}

Dolezel, Jaroslav ^{[8
]}

Coaker, Gitta ^{[9
]}

Pozniak, Curtis J. ^{[4
,5
]}

Fahima, Tzion ^{[1
,2
]}

机构：

[1] Univ Haifa, Inst Evolut, IL-3498838 Haifa, Israel

[2] Univ Haifa, Dept Evolutionary & Environm Biol, IL-3498838 Haifa, Israel

[3] Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China

[4] Univ Saskatchewan, Crop Dev Ctr, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

[5] Univ Saskatchewan, Dept Plant Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

[6] Agr Res Org ARO, Dept Vegetables, Inst Plant Sci, Volcani Ctr, IL-7505101 Rishon Leziyyon, Israel

[7] China Agr Univ, Dept Crop Genom & Bioinformat, Beijing 100094, Peoples R China

[8] Czech Acad Sci, Ctr Reg Hana Biotechnol & Agr Res, Inst Expt Bot, Slechtitel U 31, Olomouc 77900, Czech Republic

[9] Univ Calif Davis, Plant Pathol Dept, Davis, CA 95616 USA

[10] Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada

[11] ELKH, Ctr Agr Res, Agr Inst, Martonvasar, Hungary

来源：

PLANT COMMUNICATIONS | 2024年 / 5卷 / 01期

基金：

美国国家科学基金会; 以色列科学基金会;

关键词：

POWDERY MILDEW RESISTANCE; WILD EMMER WHEAT; DISEASE-RESISTANCE; BREAD WHEAT; CONFERS RESISTANCE; TETRAPLOID WHEAT; DNA; CHROMOSOMES; EVOLUTION; IDENTIFICATION;

D O I：

10.1016/j.xplc.2023.100646

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene cloning in repeat-rich polyploid genomes remains challenging. Here, we describe a strategy for overcoming major bottlenecks in cloning of the powdery mildew resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat. A conventional positional cloning approach was not effective owing to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNA sequencing reads from susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR and was discovered in only one location across the wild emmer wheat distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.

引用

页数：15

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