Exosome and lipid metabolism-related genes in pancreatic adenocarcinoma: a prognosis analysis

被引：0

作者：

Wu, Jia ^{[1
]}

Li, Yajun ^{[1
]}

Nabi, Ghulam ^{[2
]}

Huang, Xin ^{[1
]}

Zhang, Xu ^{[1
,3
]}

Wang, Yuanzhen ^{[1
]}

Huang, Liya ^{[1
]}

机构：

[1] Ningxia Med Univ, Dept Gastroenterol, Gen Hosp, Yinchuan, Ningxia, Peoples R China

[2] Polish Acad Sci, Inst Nat Conservat, Krakow, Poland

[3] Tradit Cinese Med Hosp Yinchuan, Dept Gastroenterol, Yinchuan, Ningxia, Peoples R China

来源：

AGING-US | 2023年 / 15卷 / 20期

基金：

中国国家自然科学基金;

关键词：

pancreatic adenocarcinoma; prognosis analysis; exosome; lipid metabolism; CYCLASE-ASSOCIATED PROTEIN-1; EPIDERMAL-GROWTH-FACTOR; FACTOR RECEPTOR; REGULATED EXPRESSION; R PACKAGE; CANCER; PATHWAY; METASTASIS; MIGRATION; DATABASE;

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective: The purpose of the study was to investigate the role of exosome and lipid metabolism-related genes (EALMRGs) mRNA levels in the diagnosis and prognosis of Pancreatic Adenocarcinoma (PAAD). Methods: The mRNA expression pattern of PAAD and pan-cancers with prognostic data were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. EALMRGs were acquired from GeneCards and MSigDB database after merging and deduplication. Prognostic EALMRGs were screened through univariate COX regression analysis, and a prognostic model was constructed based on these genes by least absolute shrinkage and selection operator (LASSO) regression. The prognostic value of EALMRGs was then validated in pan-cancer data. The time characteristics ROC curve analysis was performed to evaluate the effectiveness of the prognostic genes. Results: We identified 5 hub genes (ABCB1, CAP1, EGFR, PPARG, SNCA) according to high and low-risk groups of prognoses. The risk formula was verified in three other cohort of pancreatic cancer patients and was explored in pan-cancer data. Additionally, T cell and dendritic cell infiltration was significantly increased in low-risk group. The expression of the 5 hub genes was also identified in single-cell sequencing data of pancreatic cancer with pivotal pathways. Additionally, functional enrichment analysis based on pancreatic cancer data in pancreatic cancer showed that protein serine/threonine kinase activity, focal adhesion, actin binding, cell substrate junction, organic acid transport, and regulation of transporter activity were significant related to the expression of genes in EALMRGs. Conclusions: Our risk formula shows potential prognostic value in multiple cancers and manifest pivotal alterations in immune infiltration and biological pathway in pancreatic cancer.

引用

页码：11331 / 11368

页数：38

共 106 条

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