High-Throughput Antibody Profiling Identifies Targets of Protective Immunity against P. falciparum Malaria in Thailand

被引:2
作者
Hassan, Ifra [1 ]
Kanoi, Bernard N. [2 ]
Nagaoka, Hikaru [1 ]
Sattabongkot, Jetsumon [3 ]
Udomsangpetch, Rachanee [4 ]
Tsuboi, Takafumi [5 ]
Takashima, Eizo [1 ]
机构
[1] Ehime Univ, Proteo Sci Ctr, Div Malaria Res, Matsuyama 7908577, Japan
[2] Mt Kenya Univ, Inst Trop Med, Ctr Malaria Eliminat, Thika 01000, Kenya
[3] Mahidol Univ, Fac Trop Med, Mahidol Vivax Res Unit, Bangkok 10400, Thailand
[4] Mahidol Univ, Fac Med Technol, Ctr Res & Innovat, Nakhon Pathom 73170, Thailand
[5] Ehime Univ, Proteo Sci Ctr, Div Cell Free Sci, Matsuyama 7908577, Japan
关键词
malaria; wheat germ cell-free system; immunoscreening; AlphaScreen; PLASMODIUM-FALCIPARUM; PROTEINS; BINDING;
D O I
10.3390/biom13081267
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Malaria poses a significant global health challenge, resulting in approximately 600,000 deaths each year. Individuals living in regions with endemic malaria have the potential to develop partial immunity, thanks in part to the presence of anti-plasmodium antibodies. As efforts are made to optimize and implement strategies to reduce malaria transmission and ultimately eliminate the disease, it is crucial to understand how these interventions impact naturally acquired protective immunity. To shed light on this, our study focused on assessing antibody responses to a carefully curated library of P. falciparum recombinant proteins (n = 691) using samples collected from individuals residing in a low-malaria-transmission region of Thailand. We conducted the antibody assays using the AlphaScreen system, a high-throughput homogeneous proximity-based bead assay that detects protein interactions. We observed that out of the 691 variable surface and merozoite stage proteins included in the library, antibodies to 268 antigens significantly correlated with the absence of symptomatic malaria in an univariate analysis. Notably, the most prominent antigens identified were P. falciparum erythrocyte membrane protein 1 (PfEMP1) domains. These results align with our previous research conducted in Uganda, suggesting that similar antigens like PfEMP1s might play a pivotal role in determining infection outcomes in diverse populations. To further our understanding, it remains critical to conduct functional characterization of these identified proteins, exploring their potential as correlates of protection or as targets for vaccine development.
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页数:13
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共 41 条
  • [1] PRIMARY STRUCTURE AND LOCALIZATION OF A CONSERVED IMMUNOGENIC PLASMODIUM-FALCIPARUM GLUTAMATE RICH PROTEIN (GLURP) EXPRESSED IN BOTH THE PREERYTHROCYTIC AND ERYTHROCYTIC STAGES OF THE VERTEBRATE LIFE-CYCLE
    BORRE, MB
    DZIEGIEL, M
    HOGH, B
    PETERSEN, E
    RIENECK, K
    RILEY, E
    MEIS, JF
    AIKAWA, M
    NAKAMURA, K
    HARADA, M
    WIND, A
    JAKOBSEN, PH
    COWLAND, J
    JEPSEN, S
    AXELSEN, NH
    VUUST, J
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (01) : 119 - 132
  • [2] Proteome-wide analysis of a malaria vaccine study reveals personalized humoral immune profiles in Tanzanian adults
    Camponovo, Flavia
    Campo, Joseph J.
    Le, Timothy Q.
    Oberai, Amit
    Hung, Christopher
    Pablo, Jozelyn, V
    Teng, Andy A.
    Liang, Xiaowu
    Sim, B. Kim Lee
    Jongo, Said
    Abdulla, Salim
    Tanner, Marcel
    Hoffman, Stephen L.
    Daubenberger, Claudia
    Penny, Melissa A.
    [J]. ELIFE, 2020, 9 : 1 - 21
  • [3] Structural basis of malaria RIFIN binding by LILRB1-containing antibodies
    Chen, Yiwei
    Xu, Kai
    Piccoli, Luca
    Foglierini, Mathilde
    Tan, Joshua
    Jin, Wenjie
    Gorman, Jason
    Tsybovsky, Yaroslav
    Zhang, Baoshan
    Traore, Boubacar
    Silacci-Fregni, Chiara
    Daubenberger, Claudia
    Crompton, Peter D.
    Geiger, Roger
    Sallusto, Federica
    Kwong, Peter D.
    Lanzavecchia, Antonio
    [J]. NATURE, 2021, 592 (7855) : 639 - +
  • [4] Malaria prevention: from immunological concepts to effective vaccines and protective antibodies
    Cockburn, Ian A.
    Seder, Robert A.
    [J]. NATURE IMMUNOLOGY, 2018, 19 (11) : 1199 - 1211
  • [5] Infectivity of asymptomatic Plasmodium-infected human populations to Anopheles dirus mosquitoes in western Thailand
    Coleman, RE
    Kumpitak, C
    Ponlawat, A
    Maneechai, N
    Phunkitchar, V
    Rachapaew, N
    Zollner, G
    Sattabongkot, JN
    [J]. JOURNAL OF MEDICAL ENTOMOLOGY, 2004, 41 (02) : 201 - 208
  • [6] A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray
    Crompton, Peter D.
    Kayala, Matthew A.
    Traore, Boubacar
    Kayentao, Kassoum
    Ongoiba, Aissata
    Weiss, Greta E.
    Molina, Douglas M.
    Burk, Chad R.
    Waisberg, Michael
    Jasinskas, Algis
    Tan, Xiaolin
    Doumbo, Safiatou
    Doumtabe, Didier
    Kone, Younoussou
    Narum, David L.
    Liang, Xiaowu
    Doumbo, Ogobara K.
    Miller, Louis H.
    Doolan, Denise L.
    Baldi, Pierre
    Felgner, Philip L.
    Pierce, Susan K.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (15) : 6958 - 6963
  • [7] IgG acquisition against PfEMP1 PF11_0521 domain cassette DC13, DBLβ3_D4 domain, and peptides located within these constructs in children with cerebral malaria
    Cyril, Badaut
    Visitdesotrakul, Pimnitah
    Chabry, Aurelie
    Bigey, Pascal
    Tornyigah, Bernard
    Roman, Jocelyne
    Maroufou, Jules Alao
    Amoussou, Annick
    Ayivi, Blaise Serge
    Sagbo, Gratien
    Ndam, Nicaise Tuikue
    Oleinikov, Andrew V.
    Tahar, Rachida
    [J]. SCIENTIFIC REPORTS, 2021, 11 (01)
  • [8] Acquired Immunity to Malaria
    Doolan, Denise L.
    Dobano, Carlota
    Baird, J. Kevin
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2009, 22 (01) : 13 - 36
  • [9] Profiling humoral immune responses to P-falciparum infection with protein microarrays
    Doolan, Denise L.
    Mu, Yunxiang
    Unal, Berkay
    Sundaresh, Suman
    Hirst, Siddiqua
    Valdez, Conrad
    Randall, Arlo
    Molina, Douglas
    Liang, Xiaowu
    Freilich, Daniel A.
    Oloo, J. Aggrey
    Blair, Peter L.
    Aguiar, Joao C.
    Baldi, Pierre
    Davies, D. Huw
    Felgner, Philip L.
    [J]. PROTEOMICS, 2008, 8 (22) : 4680 - 4694
  • [10] Eglen Richard M, 2008, Curr Chem Genomics, V1, P2, DOI 10.2174/1875397300801010002