An Overview of the Conventional and Novel Methods Employed for SARS-CoV-2 Neutralizing Antibody Measurement

被引：7

作者：

Rocha, Vinicius Pinto Costa ^{[1
,2
]}

Quadros, Helenita Costa ^{[2
]}

Fernandes, Antonio Marcio Santana ^{[1
]}

Goncalves, Luana Pereira ^{[1
]}

Badaro, Roberto Jose da Silva ^{[1
]}

Soares, Milena Botelho Pereira ^{[1
,2
]}

Machado, Bruna Aparecida Souza ^{[1
]}

机构：

[1] SENAI CIMATEC, Inst Hlth Technol, Natl Ind Learning Serv Integrated Mfg & Technol Ca, BR-41650010 Salvador, BA, Brazil

[2] Fiocruz MS, Oswaldo Cruz Fdn, Lab Tissue Engn & Immunopharmacol, Goncalo Moniz Inst, BR-40296710 Salvador, BA, Brazil

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 07期

关键词：

neutralization test; neutralizing antibodies; COVID-19; SARS-CoV-2; MESSENGER-RNA VACCINE; BNT162B2; OMICRON; ASSAY; IMMUNOGENICITY; COVID-19;

D O I：

10.3390/v15071504

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

SARS-CoV-2 is the etiological agent of the coronavirus disease-19 (COVID-19) and is responsible for the pandemic that started in 2020. The virus enters the host cell through the interaction of its spike glycoprotein with the angiotensin converting enzyme-2 (ACE2) on the host cell's surface. Antibodies present an important role during the infection and pathogenesis due to many reasons, including the neutralization of viruses by binding to different spike epitopes. Therefore, measuring the neutralizing antibody titers in the whole population is important for COVID-19's epidemiology. Different methods are described in the literature, and some have been used to validate the main vaccines used worldwide. In this review, we discuss the main methods used to quantify neutralizing antibody titers, their advantages and limitations, as well as new approaches to determineACE2/spike blockage by antibodies.

引用

页数：18

共 81 条

[1] A simple protein-based surrogate neutralization assay for SARS-CoV-2 [J].

Abe, Kento T. ;

Li, Zhijie ;

Samson, Reuben ;

Samavarchi-Tehrani, Payman ;

Valcourt, Emelissa J. ;

Wood, Heidi ;

Budylowski, Patrick ;

Dupuis, Alan P. ;

Girardin, Roxie C. ;

Rathod, Bhavisha ;

Wang, Jenny H. ;

Barrios-Rodiles, Miriam ;

Colwill, Karen ;

McGeer, Allison J. ;

Mubareka, Samira ;

Gommerman, Jennifer L. ;

Durocher, Yves ;

Ostrowski, Mario ;

McDonough, Kathleen A. ;

Drebot, Michael A. ;

Drews, Steven J. ;

Rini, James M. ;

Gingras, Anne-Claude .

JCI INSIGHT, 2020, 5 (19)

[2] Evaluation of mRNA-1273 SARS-CoV-2 Vaccine in Adolescents [J].

Ali, Kashif ;

Berman, Gary ;

Zhou, Honghong ;

Deng, Weiping ;

Faughnan, Veronica ;

Coronado-Voges, Maria ;

Ding, Baoyu ;

Dooley, Jacqueline ;

Girard, Bethany ;

Hillebrand, William ;

Pajon, Rolando ;

Miller, Jacqueline M. ;

Leav, Brett ;

McPhee, Roderick .

NEW ENGLAND JOURNAL OF MEDICINE, 2021, 385 (24) :2241-2251

[3] Immunogenicity of Ad26.COV2.S vaccine against SARS-CoV-2 variants in humans [J].

Alter, Galit ;

Yu, Jingyou ;

Liu, Jinyan ;

Chandrashekar, Abishek ;

Borducchi, Erica N. ;

Tostanoski, Lisa H. ;

McMahan, Katherine ;

Jacob-Dolan, Catherine ;

Martinez, David R. ;

Chang, Aiquan ;

Anioke, Tochi ;

Lifton, Michelle ;

Nkolola, Joseph ;

Stephenson, Kathryn E. ;

Atyeo, Caroline ;

Shin, Sally ;

Fields, Paul ;

Kaplan, Ian ;

Robins, Harlan ;

Amanat, Fatima ;

Krammer, Florian ;

Baric, Ralph S. ;

Le Gars, Mathieu ;

Sadoff, Jerald ;

de Groot, Anne Marit ;

Heerwegh, Dirk ;

Struyf, Frank ;

Douoguih, Macaya ;

van Hoof, Johan ;

Schuitemaker, Hanneke ;

Barouch, Dan H. .

NATURE, 2021, 596 (7871) :268-+

[4] Safety and Immunogenicity of SARS-CoV-2 mRNA-1273 Vaccine in Older Adults [J].

Anderson, E. J. ;

Rouphael, N. G. ;

Widge, A. T. ;

Jackson, L. A. ;

Roberts, P. C. ;

Makhene, M. ;

Chappell, J. D. ;

Denison, M. R. ;

Stevens, L. J. ;

Pruijssers, A. J. ;

McDermott, A. B. ;

Flach, B. ;

Lin, B. C. ;

Doria-Rose, N. A. ;

O'Dell, S. ;

Schmidt, S. D. ;

Corbett, K. S. ;

Swanson, P. A., II ;

Padilla, M. ;

Neuzil, K. M. ;

Bennett, H. ;

Leav, B. ;

Makowski, M. ;

Albert, J. ;

Cross, K. ;

Edara, V. V. ;

Floyd, K. ;

Suthar, M. S. ;

Martinez, D. R. ;

Baric, R. ;

Buchanan, W. ;

Luke, C. J. ;

Phadke, V. K. ;

Rostad, C. A. ;

Ledgerwood, J. E. ;

Graham, B. S. ;

Beigel, J. H. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (25) :2427-2438

[5] Presence and short-term persistence of SARS-CoV-2 neutralizing antibodies in COVID-19 convalescent plasma donors [J].

Annen, Kyle ;

Morrison, Thomas E. ;

DomBourian, Melkon G. ;

McCarthy, Mary K. ;

Huey, Leah ;

Merkel, Patricia A. ;

Andersen, Gillian ;

Schwartz, Eileen ;

Knight, Vijaya .

TRANSFUSION, 2021, 61 (04) :1148-1159

[6]

[Anonymous], WHO Coronavirus (COVID-19) Dashboard

[7] Treatment and prevention strategies for the COVID 19 pandemic: A review of immunotherapeutic approaches for neutralizing SARS-CoV-2 [J].

Baral, Pravas Kumar ;

Yin, Jiang ;

James, Michael N. G. .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2021, 186 :490-500

[8] Phase 1/2 trial of SARS-CoV-2 vaccine ChAdOx1 nCoV-19 with a booster dose induces multifunctional antibody responses [J].

Barrett, Jordan R. ;

Belij-Rammerstorfer, Sandra ;

Dold, Christina ;

Ewer, Katie J. ;

Folegatti, Pedro M. ;

Gilbride, Ciaran ;

Halkerston, Rachel ;

Hill, Jennifer ;

Jenkin, Daniel ;

Stockdale, Lisa ;

Verheul, Marije K. ;

Aley, Parvinder K. ;

Angus, Brian ;

Bellamy, Duncan ;

Berrie, Eleanor ;

Bibi, Sagida ;

Bittaye, Mustapha ;

Carroll, Miles W. ;

Cavell, Breeze ;

Clutterbuck, Elizabeth A. ;

Edwards, Nick ;

Flaxman, Amy ;

Fuskova, Michelle ;

Gorringe, Andrew ;

Hallis, Bassam ;

Kerridge, Simon ;

Lawrie, Alison M. ;

Linder, Aline ;

Liu, Xinxue ;

Madhavan, Meera ;

Makinson, Rebecca ;

Mellors, Jack ;

Minassian, Angela ;

Moore, Maria ;

Mujadidi, Yama ;

Plested, Emma ;

Poulton, Ian ;

Ramasamy, Maheshi N. ;

Robinson, Hannah ;

Rollier, Christine S. ;

Song, Rinn ;

Snape, Matthew D. ;

Tarrant, Richard ;

Taylor, Stephen ;

Thomas, Kelly M. ;

Voysey, Merryn ;

Watson, Marion E. E. ;

Wright, Daniel ;

Douglas, Alexander D. ;

Green, Catherine M. .

NATURE MEDICINE, 2021, 27 (02) :279-+

[9] Biomimetic nanoplasmonic sensor for rapid evaluation of neutralizing SARS-CoV-2 monoclonal antibodies as antiviral therapy [J].

Batool, Razia ;

Soler, Maria ;

Colavita, Francesca ;

Fabeni, Lavinia ;

Matusali, Giulia ;

Lechuga, Laura M. .

BIOSENSORS & BIOELECTRONICS, 2023, 226

[10] REGN-COV2 antibodies prevent and treat SARS-CoV-2 infection in rhesus macaques and hamsters [J].

Baum, Alina ;

Ajithdoss, Dharani ;

Copin, Richard ;

Zhou, Anbo ;

Lanza, Kathryn ;

Negron, Nicole ;

Ni, Min ;

Wei, Yi ;

Mohammadi, Kusha ;

Musser, Bret ;

Atwal, Gurinder S. ;

Oyejide, Adelekan ;

Goez-Gazi, Yenny ;

Dutton, John ;

Clemmons, Elizabeth ;

Staples, Hilary M. ;

Bartley, Carmen ;

Klaffke, Benjamin ;

Alfson, Kendra ;

Gazi, Michal ;

Gonzalez, Olga ;

Dick, Edward, Jr. ;

Carrion, Ricardo, Jr. ;

Pessaint, Laurent ;

Porto, Maciel ;

Cook, Anthony ;

Brown, Renita ;

Ali, Vaneesha ;

Greenhouse, Jack ;

Taylor, Tammy ;

Andersen, Hanne ;

Lewis, Mark G. ;

Stahl, Neil ;

Murphy, Andrew J. ;

Yancopoulos, George D. ;

Kyratsous, Christos A. .

SCIENCE, 2020, 370 (6520) :1110-+

← 1 2 3 4 5 6 7 8 9 →