High-Yield Characterization of Single Molecule Interactions with DeepTip™ Atomic Force Microscopy Probes

被引：3

作者：

Corregidor, Daniel ^{[1
,2
]}

Tabraue, Raquel ^{[1
,2
]}

Colchero, Luis ^{[1
,2
]}

Daza, Rafael ^{[1
,2
]}

Elices, Manuel ^{[1
,2
]}

Guinea, Gustavo, V ^{[1
,2
,3
,4
]}

Perez-Rigueiro, Jose ^{[1
,2
,3
,4
]}

机构：

[1] Univ Politecn Madrid, Ctr Tecnol Biomed, Madrid 28223, Spain

[2] Univ Politecn Madrid, Dept Ciencia Mat, ETSI Caminos Canales & Puertos, Madrid 28040, Spain

[3] Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed CI, Madrid 28029, Spain

[4] Inst Invest Sanitaria Hosp Clin San Carlos IdISSC, Biomat & Regenerat Med Grp, Calle Prof Martin Lagos S-N, Madrid 28040, Spain

来源：

MOLECULES | 2023年 / 28卷 / 01期

关键词：

affinity atomic force microscopy; AFM; streptavidin; biotin; functionalization; ENERGY LANDSCAPE; BIOTIN; AFM; SPECTROSCOPY; FUNCTIONALIZATION; IMMOBILIZATION; STRATEGIES; ANTIBODIES; LIGANDS; SURFACE;

D O I：

10.3390/molecules28010226

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTip (TM) probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular events. The procedure comprises the covalent binding of the target molecule to a surface and of the sensing molecule to the DeepTip (TM) probe, so that the interaction between both chemical species can be characterized by obtaining force-displacement curves in an atomic force microscope. It is shown that molecular resolution is consistently attained with a percentage of successful events higher than 90% of the total number of recorded curves, and a very low level of unspecific interactions. The combination of both features is a clear indication of the robustness and versatility of the proposed methodology.

引用

页数：13

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