Evaluation of Rap1GAP and EPAC1 Gene Expression in Endometriosis Disease

被引:2
作者
Dehghanian, Mehran [1 ]
Yarahmadi, Ghafour [1 ]
Sandoghsaz, Reyhaneh Sadat [2 ]
Khodadadian, Ali [1 ]
Shamsi, Farimah [3 ]
Mehrjardi, Mohammad Yahya Vahidi [4 ]
机构
[1] Shahid Sadoughi Univ Med Sci, Dept Med Genet, Yazd, Iran
[2] Yazd Reprod Sci Inst, Shahid Sadoughi Univ Med Sci, Abort Res Ctr, Yazd, Iran
[3] Shahid Sadoughi Univ Med Sci, Sch Pulic Hlth, Dept Biostat & Epidemiol, Ctr Healthcare Data Modeling, Yazd, Iran
[4] Shahid Sadoughi Univ Med Sci, Sch Publ Hlth, Res Ctr Food Hyg & Safety, Yazd, Iran
来源
ADVANCED BIOMEDICAL RESEARCH | 2023年 / 12卷 / 01期
关键词
Endometriosis; EPAC1; gene expression; Rap1GAP; DOWN-REGULATION; IN-VITRO; PROLIFERATION; MIGRATION; CELLS;
D O I
10.4103/abr.abr_86_22
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background: Endometriosis is a female reproductive system disease in which the endometrial tissue is found in other women's organs. Various factors are effective in the development of endometriosis, and because of the interaction of genetics and environmental factors, this disease is a multi-factorial disease. MAPK/ERK and PI3K/Akt/mTOR pathways are activated by growth factors and steroid hormones and are known as two important pathways involved in the processes of growth, proliferation, and survival of endometriosis cells. Raps, monomeric GTPase of the Ras family, are able to activate these pathways independent of Ras. The goal of our study was to evaluate the expression level of Rap1GAP and EPAC1 genes as two important RapGAPs (GTPase-activating proteins) and RapGEFs (guanine nucleotide exchange factors), respectively, in endometriosis tissues and normal endometrium tissues. Materials and Methods: In this study, 15 samples of women without signs of endometriosis were taken as control samples. Fifteen ectopic and 15 eutopic samples were taken from women with endometriosis using laparoscopic surgery. The expression of EPAC1 and Rap1GAP genes was investigated by the real-time polymerase chain reaction technique, and the results were analyzed by one-way ANOVA test. Results: EPAC1 upregulated significantly in ectopic tissues compared to eutopic and control tissues. Rap1GAP expression was lower in ectopic tissues compared to control and eutopic tissues. Conclusions: Based on these results, it may be concluded that changes in the expression of the Rap1GAP and Epca1 genes may play a role in the pathways involved in the pathogenesis, displacement, and migration of endometriosis cells.
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相关论文
共 25 条
[1]  
Alimi Yusuf, 2018, Cureus, V10, pe3361, DOI 10.7759/cureus.3361
[2]   The role of mitogen-activated protein kinase signaling pathway in endometriosis [J].
Bora, Gizem ;
Yaba, Aylin .
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 2021, 47 (05) :1610-1623
[3]   Evaluation of the relationship between miR-337-3p and RAPIA gene in endometriosis [J].
Dehghanian, Mehran ;
Yarahmadi, Ghafour ;
Fazeli, Javad ;
Vahidi Mehrjardi, Mohammad Yahya ;
Javaheri, Atiyeh ;
Kalantar, Seyed Mehdi ;
Dehghani, Mohammadreza .
JOURNAL OF ENDOMETRIOSIS AND PELVIC PAIN DISORDERS, 2022, 14 (04) :178-182
[4]   Rap1a deficiency modifies cytokine responses and MAPK-signaling in vitro and impairs the in vivo inflammatory response [J].
Dorn, Annette ;
Zoellner, Anna ;
Follo, Marie ;
Martin, Stefan ;
Weber, Felix ;
Marks, Reinhard ;
Melchinger, Wolfgang ;
Zeiser, Robert ;
Fisch, Paul ;
Scheele, Juergen S. .
CELLULAR IMMUNOLOGY, 2012, 276 (1-2) :187-195
[5]   RAP1GAP Functions as a Tumor Suppressor Gene and Is Regulated by DNA Methylation in Differentiated Thyroid Cancer [J].
Faam, Bita ;
Ghaffari, Mohammad A. ;
Khorsandi, Layasadat ;
Ghadiri, Ata A. ;
Totonchi, Mehdi ;
Amouzegar, Atieh ;
Fanaei, S. Ahmad ;
Azizi, Fereidoun ;
Shahbazian, Hajeih B. ;
Hashemi Tabar, Mahmoud .
CYTOGENETIC AND GENOME RESEARCH, 2021, 161 (05) :227-235
[6]   Role of EPAC1 Signalosomes in Cell Fate: Friends or Foes? [J].
Formoso, Karina ;
Lezoualc'h, Frank ;
Mialet-Perez, Jeanne .
CELLS, 2020, 9 (09)
[7]   Epac1 knockdown inhibits the proliferation of ovarian cancer cells by inactivating AKT/Cyclin D1/CDK4 pathway in vitro and in vivo [J].
Gao, Meng ;
Ma, Yanyan ;
Bast, Robert C., Jr. ;
Li, Yue ;
Wan, Lu ;
Liu, Yanping ;
Sun, Yingshuo ;
Fang, Zhenghui ;
Zhang, Lining ;
Wang, Xiaoyan ;
Wei, Zengtao .
MEDICAL ONCOLOGY, 2016, 33 (07)
[8]   The Many Faces of Rap1 GTPase [J].
Jaskiewicz, Anna ;
Pajak, Beata ;
Orzechowski, Arkadiusz .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (10)
[9]   RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway [J].
Jin, Xin ;
Di, Xin ;
Wang, Ruimin ;
Ma, He ;
Tian, Chang ;
Zhao, Min ;
Cong, Shan ;
Liu, Jiaying ;
Li, Ranwei ;
Wang, Ke .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2019, 23 (06) :3897-3904
[10]   Expression profiling of endometrium from women with endometriosis reveals candidate genes for disease-based implantation failure and infertility [J].
Kao, LC ;
Germeyer, A ;
Tulac, S ;
Lobo, S ;
Yang, JP ;
Taylor, RN ;
Osteen, K ;
Lessey, BA ;
Giudice, LC .
ENDOCRINOLOGY, 2003, 144 (07) :2870-2881