Complete genome sequence of a novel double-stranded RNA virus infecting the phytopathogenic fungus Rhizopus stolonifer

被引：2

作者：

Wang, Hao ^{[1
,2
]}

Luo, Jingxian ^{[1
,2
]}

Dai, Ruoyin ^{[1
,2
]}

Shah, Kaleem Ullah ^{[1
,2
]}

Andika, Ida Bagus ^{[3
]}

Sun, Liying ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Peoples R China

[2] Northwest A&F Univ, Coll Plant Protect, Yangling, Peoples R China

[3] Qingdao Agr Univ, Coll Plant Hlth & Med, Qingdao, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2023年 / 168卷 / 09期

基金：

中国国家自然科学基金;

关键词：

mycovirus; Totiviridae; Rhizopus stolonifer; Zygomycota; Fungi; DNA MYCOVIRUS; PLANT; MYCOREOVIRUS;

D O I：

10.1007/s00705-023-05869-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we report the complete genome sequence of a novel toti-like virus, tentatively named "Rhizopus stolonifer double-stranded RNA virus 1" (RsDSV1), identified from a phytopathogenic fungal agent of apple fruit rot disease, Rhizopus stolonifer strain A2-1. RsDSV1 has a double-stranded RNA genome. The complete RsDSV1 genome is 5178 nucleotides (nt) in length and contains two open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on RdRp and CP amino acid sequences revealed that RsDSV1 is closely related to unclassified members of the family Totiviridae. In stress-inducing Vogel's minimal and sodium dodecyl sulfate-containing media, hyphal growth of A2-1 was suppressed, but the accumulation of RsDSV1 RNA increased, indicating that stresses promote RsDSV1 replication. To our knowledge, this is the first report of a mycovirus found in R. stolonifer.

引用

页数：7

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