Spike-specific T cells are enriched in breastmilk following SARS-CoV-2 mRNA vaccination

被引：13

作者：

Armistead, Blair ^{[1
]}

Jiang, Yonghou ^{[1
]}

Carlson, Marc ^{[2
]}

Ford, Emily S. ^{[3
,4
]}

Jani, Saumya ^{[4
,5
]}

Houck, John ^{[1
]}

Wu, Xia ^{[4
]}

Jing, Lichen ^{[4
]}

Pecor, Tiffany ^{[1
]}

Kachikis, Alisa ^{[6
]}

Yeung, Winnie ^{[1
]}

Nguyen, Tina ^{[1
]}

Coig, Rene ^{[5
]}

Minkah, Nana ^{[1
,7
]}

Larsen, Sasha E. ^{[1
]}

Coler, Rhea N. ^{[1
,7
,8
]}

Koelle, David M. ^{[3
,4
,5
,8
,9
]}

Harrington, Whitney E. ^{[1
,7
,8
]}

机构：

[1] Seattle Childrens Res Inst, Ctr Global Infect Dis Res, Seattle, WA 98105 USA

[2] Seattle Childrens Res Inst, Res Sci Comp, Enterprise Analyt, Seattle, WA USA

[3] Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, Seattle, WA USA

[4] Univ Washington, Dept Med, Seattle, WA USA

[5] Univ Washington, Dept Lab Med & Pathol, Seattle, WA USA

[6] Univ Washington, Dept Obstet & Gynecol, Seattle, WA USA

[7] Univ Washington, Dept Pediat, Seattle, WA 98195 USA

[8] Univ Washington, Dept Global Hlth, Seattle, WA 98195 USA

[9] Benaroya Res Inst, Seattle, WA USA

来源：

MUCOSAL IMMUNOLOGY | 2023年 / 16卷 / 01期

关键词：

IMMUNOMODULATORY CONSTITUENTS; INTESTINAL PERMEABILITY; RESPONSES; REPERTOIRES; LYMPHOCYTES;

D O I：

10.1016/j.mucimm.2023.01.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human breastmilk is rich in T cells; however, their specificity and function are largely unknown. We compared the phenotype, diversity, and antigen specificity of T cells in breastmilk and peripheral blood of lactating individuals who received SARS-CoV-2 messenger RNA (mRNA) vaccination. Relative to blood, breastmilk contained higher frequencies of T effector and central memory populations that expressed mucosal-homing markers. T cell receptor sequence overlap was limited between blood and breastmilk. Overabundant breastmilk clones were observed in all individuals, were diverse, and contained complementarity-determining regions in three sequences with known epitope specificity, including to SARS-CoV-2 spike. SARS-CoV-2 spike-specific T cell receptors were more frequent in breastmilk compared to blood and expanded in breastmilk following a 3rd mRNA vaccine dose. Our observations indicate that the lactating breast contains a distinct T cell population that can be modulated by maternal vaccination with potential implications for passive infant protection.

引用

页码：39 / 49

页数：11

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