In vitro gastrointestinal digestion and colonic fermentation of media-milled black rice particle-stabilized Pickering emulsion: Phenolic release, bioactivity and prebiotic potential

This is a pioneer study that investigated the digestive characteristics of Pickering emulsions stabilized by mediamilled black rice particles during in vitro digestion and colonic fermentation. Free fatty acid release of the emulsions improved from 28.42 & PLUSMN; 3.13% to 33.68 & PLUSMN; 4.05% after media milling. The phenolics released from media-milled sample were close to those from unground sample. Media-milled sample exhibited higher DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging ability and & alpha;-glucosidase inhibition rate than unground sample. Media milling increased the generation of short-chain fatty acids (SCFAs) during colonic fermentation, especially acetic acid (23% improvement in media-milled sample over unground sample). It also inhibited the growth of harmful bacteria namely Escherichia Shigella and Streptococcus, and promoted the growth of beneficial bacteria including Bifidobacterium and Blautia. These findings revealed that media-milled black rice particlestabilized Pickering emulsions possessed intrinsic bioactivity and prebiotic potentials in the gastrointestinal tract for the first time.