The Characteristics of Bacillus cereus Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks during Climate Change

被引：1

作者：

Goncharova, Y. O. ^{[1
]}

Evseeva, V. V. ^{[1
]}

Mironova, R. I. ^{[1
]}

Khlopova, K. V. ^{[1
]}

Bogun, A. G. ^{[1
]}

Sizova, A. A. ^{[1
]}

Solomentsev, V. I. ^{[1
]}

Titareva, G. M. ^{[1
]}

Bahtejeva, I. V. ^{[1
]}

Kravchenko, T. B. ^{[1
]}

Brushkov, A. V. ^{[2
,3
]}

Timofeev, V. S. ^{[1
]}

Ignatov, S. G. ^{[1
,2
]}

机构：

[1] State Res Ctr Appl Microbiol & Biotechnol, Obolensk 142279, Moscow Oblast, Russia

[2] Moscow MV Lomonosov State Univ, Moscow 119991, Russia

[3] Tyumen State Univ, Tyumen 625003, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2023年 / 59卷 / 06期

关键词：

Bacillus cereus group; Bacillus anthracis; toxins; genotyping; MLVA; MLST; RAPD; permafrost; POPULATION-STRUCTURE; ANTHRACIS; IDENTIFICATION; THURINGIENSIS; MEMBERS; TOXIN; ASSAY; PCR;

D O I：

10.1134/S0003683823060054

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Strains of Bacillus genus were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the Bacillus cereus complex. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differentiate the studied strains from the B. anthracis species and to show their intraspecific genetic differences and the degree of relationship. Whole genome sequencing was carried out, based on the data of which MLST genotyping was carried out, which revealed two known sequence types and one new one that is described for the first time in this work. The results we obtained are of practical importance and are extremely interesting from the point of view of the evolution and phylogeography of the B. cereus group, since the fact that strains were isolated from permafrost suggests that their age may be much older than expected.

引用

页码：808 / 817

页数：10

共 40 条

[1] Bacillus cereus Biovar Anthracis Causing Anthrax in Sub-Saharan Africa-Chromosomal Monophyly and Broad Geographic Distribution [J].

Antonation, Kym S. ;

Gruetzmacher, Kim ;

Dupke, Susann ;

Mabon, Philip ;

Zimmermann, Fee ;

Lankester, Felix ;

Peller, Tianna ;

Feistner, Anna ;

Todd, Angelique ;

Herbinger, Ilka ;

de Nys, Helene M. ;

Muyembe-Tamfun, Jean-Jacques ;

Karhemere, Stomy ;

Wittig, Roman M. ;

Couacy-Hymann, Emmanuel ;

Grunow, Roland ;

Calvignac-Spencer, Sebastien ;

Corbett, Cindi R. ;

Klee, Silke R. ;

Leendertz, Fabian H. .

PLOS NEGLECTED TROPICAL DISEASES, 2016, 10 (09)

[2] You Can'tB. cereus- A Review ofBacillus cereusStrains That Cause Anthrax-Like Disease [J].

Baldwin, Victoria M. .

FRONTIERS IN MICROBIOLOGY, 2020, 11

[3] Characterization of a Bacillus cereus strain associated with a large feed-related outbreak of severe infection in pigs [J].

Calvigioni, Marco ;

Cara, Alice ;

Celandroni, Francesco ;

Mazzantini, Diletta ;

Panattoni, Adelaide ;

Tirloni, Erica ;

Bernardi, Cristian ;

Pinotti, Luciano ;

Stella, Simone ;

Ghelardi, Emilia .

JOURNAL OF APPLIED MICROBIOLOGY, 2022, 133 (02) :1078-1088

[4] Rapid, High-Throughput Identification of Anthrax-Causing and Emetic Bacillus cereus Group Genome Assemblies via BTyper, a Computational Tool for Virulence-Based Classification of Bacillus cereus Group Isolates by Using Nucleotide Sequencing Data [J].

Carroll, Laura M. ;

Kovac, Jasna ;

Miller, Rachel A. ;

Wiedmann, Martin .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2017, 83 (17)

[5] Using average nucleotide identity to improve taxonomic assignments in prokaryotic genomes at the NCBI [J].

Ciufo, Stacy ;

Kannan, Sivakumar ;

Sharma, Shobha ;

Badretdin, Azat ;

Clark, Karen ;

Turner, Sean ;

Brover, Slava ;

Schoch, Conrad L. ;

Kimchi, Avi ;

DiCuccio, Michael .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2018, 68 (07) :2386-2392

[6]

Daffonchio D, 1999, APPL ENVIRON MICROB, V65, P1298

[7] The Food Poisoning Toxins of Bacillus cereus [J].

Dietrich, Richard ;

Jessberger, Nadja ;

Ehling-Schulz, Monika ;

Maertlbauer, Erwin ;

Granum, Per Einar .

TOXINS, 2021, 13 (02)

[8]

Drean P, 2015, METHODS MOL BIOL, V1301, P71, DOI 10.1007/978-1-4939-2599-5_7

[9] Toxin gene profiling of enterotoxic and emetic Bacillus cereus [J].

Ehling-Schulz, Monika ;

Guinebretiere, Marie-Helene ;

Monthan, Amanda ;

Berge, Odile ;

Fricker, Martina ;

Svensson, Birgitta .

FEMS MICROBIOLOGY LETTERS, 2006, 260 (02) :232-240

[10] Meeting report: GenBank microbial genomic taxonomy workshop (12-13 May, 2015) [J].

Federhen, Scott ;

Rossello-Mora, Ramon ;

Klenk, Hans-Peter ;

Tindall, Brian J. ;

Konstantinidis, Konstantinos T. ;

Whitman, William B. ;

Brown, Daniel ;

Labeda, David ;

Ussery, David ;

Garrity, George M. ;

Colwell, Rita R. ;

Hasan, Nur ;

Graf, Joerg ;

Parte, Aidan ;

Yarza, Pablo ;

Goldberg, Brittany ;

Sichtig, Heike ;

Karsch-Mizrachi, Ilene ;

Clark, Karen ;

McVeigh, Richard ;

Pruitt, Kim D. ;

Tatusova, Tatiana ;

Falk, Robert ;

Turner, Sean ;

Madden, Thomas ;

Kitts, Paul ;

Kimchi, Avi ;

Klimke, William ;

Agarwala, Richa ;

DiCuccio, Michael ;

Ostell, James .

STANDARDS IN GENOMIC SCIENCES, 2016, 11

← 1 2 3 4 →