Laparoscopic embryo transfer in pigs-comparison of different variants and efficiencies of the method

被引:2
作者
Wieczorek, J. [1 ]
Stodolak-Zych, E. [2 ]
Okon, K. [3 ]
Koseniuk, J. [4 ]
Bryla, M. [5 ]
Jura, J. [5 ]
Poniedzialek-Kempny, K. [5 ]
Rajska, I. [5 ]
Sobol, K. [5 ]
Balak, M. Kotula [1 ]
Chmurska-Gasowska, M. [1 ]
机构
[1] Agr Univ Krakow, Univ Ctr Vet Med, Mickiewicza 24-28, PL-30059 Krakow, Poland
[2] Univ Sci & Technol, Fac Mat Sci & Ceram, Dept Biomat, Mickiewicza 30, PL-30059 Krakow, Poland
[3] Jagiellonian Univ, Chair Pathomorphol, Coll Med, Grzegorzecka 16, PL-30526 Krakow, Poland
[4] Artvimed Ctr Reprod Med, Czyzowka 14, PL-30526 Krakow, Poland
[5] Natl Res Inst Anim Prod, Dept Reprod Biotechnol & Cryoconservat, Krakowska 1, PL-30322 Balice, Poland
来源
POLISH JOURNAL OF VETERINARY SCIENCES | 2023年 / 26卷 / 02期
关键词
laparoscopy; embryo; transfer; pig; IN-VIVO; BLASTOCYSTS; COLLECTION; APOPTOSIS;
D O I
10.24425/pjvs.2023.145036
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The aim of the study was to develop a method of laparoscopic embryo transfer in pigs and to compare different variants of this method. Two catheter diameters (1.6 mm and 1.0 mm), the method and site of embryo deposition (oviduct or uterus), the embryo development stage (2 - 4 cell or blastocyst), the method for oviduct or uterus stabilization, the potential for cryopre-served embryo transfer, the developmental potential of the embryos after transfer to the oviduct, patomorphology of the oviduct after transfer and possible clinical complications were taken into consideration. Two studies compared two variants of transfer to the uterus, and five variants of transfer to the fallopian tube. The transfer of embryos by the infundibulum may be of limited use due to handling problems and very low efficiency (pregnancy was not achieved). Very low efficiency was shown after transfer of vitrified embryos. Transfer to the fallopian tube by punc-ture of the fallopian tube, regardless of the developmental stage of the embryo, is the recommen-ded method of embryo transfer. The histopathological examination of the fallopian tube revealed possible changes within the puncture site. The numerous clinical complications observed did not affect the effectiveness of the method.
引用
收藏
页码:295 / 306
页数:12
相关论文
共 38 条
[1]   Endoscopic embryo collection and embryo transfer into the oviduct and the uterus of pigs [J].
Besenfelder, U ;
Modl, J ;
Muller, M ;
Brem, G .
THERIOGENOLOGY, 1997, 47 (05) :1051-1060
[2]   Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos [J].
Bryla, M. ;
Trzcinska, M. ;
Wieczorek, J. ;
Slomski, R. ;
Smorag, Z. .
ANIMAL REPRODUCTION SCIENCE, 2010, 118 (01) :77-82
[3]   RELATIONSHIP BETWEEN APOPTOTIC-LIKE CHANGES IN STORED BOAR SEMEN AND DNA FRAGMENTATION IN PREIMPLANTATION EMBRYOS [J].
Bryla, Magdalena ;
Trzcinska, Monika .
ANNALS OF ANIMAL SCIENCE, 2012, 12 (03) :357-366
[4]  
Bryla M, 2011, ANN ANIM SCI, V11, P261
[5]  
Bryla M, 2009, ANIM SCI PAP REP, V27, P59
[6]  
Cao Zheng Cao Zheng, 2016, Journal of Northeast Agricultural University (English Edition), V23, P52
[7]   Uterine NK cells and macrophages in pregnancy [J].
Faas, Marijke M. ;
de Vos, Paul .
PLACENTA, 2017, 56 :44-52
[8]  
Gadea J., 2020, Reproductive Technologies in Animals, V1st ed., P67, DOI DOI 10.1016/B978-0-12-817107-3.00005-9
[9]  
Gajda B, 2004, MED WETER, V60, P371
[10]  
Gajda B, 2008, Patent, Patent No. 199290