Superior rat wound-healing activity of green synthesized silver nanoparticles from acetonitrile extract of Juglans regia L: Pellicle and leaves

The process of wound healing is complicated. Antimicrobial silver has been one of the substances used for wounds since ancient times. Moreover, traditional medicine has long used Juglans regia L. to promote wound healing. Since eco-friendly nanotechnology has various uses in biomedical research, the aim of this study was to assess the wound -healing capacity of bio-reduced silver nanoparticles (AgNPs). UV, DLS, TEM, and FTIR were used to characterize the prepared AgNPs. Pellicle's bioreduced AgNP (AgNP/P) has a better polydispersity index (PI) of 0.336 compared to its chemically synthesized peers, which have a PI of 0.67. Using incision and excision wound healing models, AgNPs and extracts were compared to Solcoseryl (R). Skin -breaking strength, wound contraction, epithelialization time, histology, and cytokines were all assessed. Juglans regia L. pellicle extract (P) has shown significant effectiveness in both models, as well as their bioreduced partner AgNP/P. The skin's tensile strength following AgNP/P therapy (871 g, p value < 0.05) is comparable to that after Solcoseryl (R) (928 g), both of which are significantly better than AgNP (592 g) in the incision wound model. Epithelialization time (16.0 and 16.5 days) did not substantially differ from Solcoseryl (R) (15.3 days) (P value < 0.05). There was an elevated collagen content. Low levels of IL1 beta (189.0 pg/g) and high levels of TNF-alpha (1007.1 pg/g) in the case of AgNP/P suggest various cellular kinds of maturation and various wound healing structures that are evident in histopathology investigations. The bioreduced AgNP/P could find use as a pharmaceutical agent for wound healing dressings.