Characterisation and development of histopathological lesions in a guinea pig model of Mycobacterium tuberculosis infection

被引：6

作者：

Larenas-Munoz, Fernanda ^{[1
]}

Ruedas-Torres, Ines ^{[1
,2
]}

Hunter, Laura ^{[2
,3
]}

Bird, Alison ^{[2
]}

Agullo-Ros, Irene ^{[1
]}

Winsbury, Rebecca ^{[2
]}

Clark, Simon ^{[2
]}

Rayner, Emma ^{[2
]}

Salguero, Francisco J. ^{[2
]}

机构：

[1] Univ Cordoba, Dept Anat & Comparat Pathol & Toxicol, Pathol & Immunol Grp UCO PIG, Zoonosis & Enfermedades Emergentes ENZOEM U, Int Excellence Agrifood Campus CeiA3, Cordoba, Spain

[2] UK Hlth Secur Agcy UKHSA, Pathol Dept, Salisbury, England

[3] Univ Surrey, Sch Biosci & Med, Guildford, Surrey, England

来源：

FRONTIERS IN VETERINARY SCIENCE | 2023年 / 10卷

基金：

美国国家卫生研究院;

关键词：

tuberculosis; guinea pig; granuloma; pathology; animal model; immunohistochemistry; cell marker; Mycobacterium tuberculosis; MACAQUES MACACA-MULATTA; AEROSOL INFECTION; COMPARATIVE PATHOLOGY; INCREASED EXPRESSION; BOVIS; GRANULOMAS; PROTECTION; CHALLENGE; DISEASE; ANTIGEN;

D O I：

10.3389/fvets.2023.1264200

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Tuberculosis (TB) remains a very significant infectious disease worldwide. New vaccines and therapies are needed, even more crucially with the increase of multi-drug resistant Mycobacterium tuberculosis strains. Preclinical animal models are very valuable for the development of these new disease control strategies. Guinea pigs are one of the best models of TB, sharing many features with the pathology observed in human TB. Here we describe the development of TB lesions in a guinea pig model of infection. We characterise the granulomatous lesions in four developmental stages (I-IV), using histopathological analysis and immunohistochemical (IHC) techniques to study macrophages, T cells, B cells and granulocytes. The granulomas in the guinea pigs start as aggregations of macrophages and few heterophils, evolving to larger lesions showing central caseous necrosis with mineralisation and abundant acid-fast bacilli, surrounded by a rim of macrophages and lymphocytes in the outer layers of the granuloma. Multinucleated giant cells are very rare and fibrotic capsules are not formed in this animal model.

引用

页数：13

共 50 条

[1] Upregulation of IL-17A, CXCL9 and CXCL10 in Early-Stage Granulomas Induced by Mycobacterium bovis in Cattle [J].

Aranday-Cortes, E. ;

Bull, N. C. ;

Villarreal-Ramos, B. ;

Gough, J. ;

Hicks, D. ;

Ortiz-Pelaez, A. ;

Vordermeier, H. M. ;

Salguero, F. J. .

TRANSBOUNDARY AND EMERGING DISEASES, 2013, 60 (06) :525-537

[2] Protective Effect of Oral BCG and Inactivated Mycobacterium bovis Vaccines in European Badgers (Meles meles) Experimentally Infected With M. bovis [J].

Balseiro, Ana ;

Prieto, Jose Miguel ;

Alvarez, Vega ;

Lesellier, Sandrine ;

Dave, Dipesh ;

Salguero, Francisco J. ;

Sevilla, Iker A. ;

Infantes-Lorenzo, Jose Antonio ;

Garrido, Joseba M. ;

Adriaensen, Hans ;

Juste, Ramon A. ;

Barral, Marta .

FRONTIERS IN VETERINARY SCIENCE, 2020, 7

[3] Experimental tuberculosis: the role of comparative pathology in the discovery of improved tuberculosis treatment strategies [J].

Basaraba, Randall J. .

TUBERCULOSIS, 2008, 88 :S35-S47

[4] Increased expression of host iron-binding proteins precedes iron accumulation and calcification of primary lung lesions in experimental tuberculosis in the guinea pig [J].

Basaraba, Randall J. ;

Bielefeldt-Ohmann, Helle ;

Eschelbach, Ellie K. ;

Reisenhauer, Claire ;

Tolnay, Airn E. ;

Taraba, Lauren C. ;

Shanley, Crystal A. ;

Smith, Erin A. ;

Bedwell, Cathy L. ;

Chlipala, Elizabeth A. ;

Ormea, Ian M. .

TUBERCULOSIS, 2008, 88 (01) :69-79

[5] Lymphadenitis as a major element of disease in the guinea pig model of tuberculosis [J].

Basaraba, Randall J. ;

Dailey, Deanna D. ;

McFarland, Christine T. ;

Shanley, Crystal A. ;

Smith, Erin E. ;

McMurray, David N. ;

Orme, Ian M. .

TUBERCULOSIS, 2006, 86 (05) :386-394

[6] Pulmonary lymphatics are primary sites of Mycobacterium tuberculosis infection in guinea pigs infected by aerosol [J].

Basaraba, Randall J. ;

Smith, Erin E. ;

Shanley, Crystal A. ;

Orme, Ian M. .

INFECTION AND IMMUNITY, 2006, 74 (09) :5397-5401

[7]

Bermejo MC, 2007, AN SIST SANIT NAVAR, V30, P7

[8] Susceptibility of Mycobacterium tuberculosis-infected host cells to phospho-MLKL driven necroptosis is dependent on cell type and presence of TNFα [J].

Butler, Rachel E. ;

Krishnan, Nitya ;

Garcia-Jimenez, Waldo ;

Francis, Robert ;

Martyn, Abbe ;

Mendum, Tom ;

Felemban, Shaza ;

Locker, Nicolas ;

Salguero, Francisco J. ;

Robertson, Brian ;

Stewart, Graham R. .

VIRULENCE, 2017, 8 (08) :1820-1832

[9] The Effect of Oral Vaccination with Mycobacterium bovis BCG on the Development of Tuberculosis in Captive European Badgers (Meles meles) [J].

Chambers, Mark A. ;

Aldwell, Frank ;

Williams, Gareth A. ;

Palmer, Si ;

Gowtage, Sonya ;

Ashford, Roland ;

Dalley, Deanna J. ;

Dave, Dipesh ;

Weyer, Ute ;

Salguero, Francisco J. ;

Nunez, Alejandro ;

Nadian, Allan K. ;

Crawshaw, Timothy ;

Corner, Leigh A. L. ;

Lesellier, Sandrine .

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2017, 7

[10] Survival of Mycobacterium tuberculosis during experimental aerosolization and implications for aerosol challenge models [J].

Clark, S. O. ;

Hall, Y. ;

Kelly, D. L. F. ;

Hatch, G. J. ;

Williams, A. .

JOURNAL OF APPLIED MICROBIOLOGY, 2011, 111 (02) :350-359

← 1 2 3 4 5 →