Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing

被引：8

作者：

Okumura, Yuichi ^{[1
,2
]}

Inomata, Takenori ^{[1
,2
,3
,4
]}

Fujimoto, Keiichi ^{[1
,2
]}

Fujio, Kenta ^{[1
,2
]}

Zhu, Jun ^{[1
]}

Yanagawa, Ai ^{[2
]}

Shokirova, Hurramhon ^{[1
]}

Saita, Yoshitomo ^{[5
,6
]}

Kobayashi, Yohei ^{[5
,6
]}

Nagao, Masahi ^{[5
,6
,7
]}

Nishio, Hirofumi ^{[5
,6
]}

Sung, Jaemyoung ^{[1
,2
,8
]}

Midorikawa-Inomata, Akie ^{[3
]}

Eguchi, Atsuko ^{[3
]}

Nagino, Ken ^{[1
,2
,3
]}

Akasaki, Yasutsugu ^{[1
,2
]}

Hirosawa, Kunihiko ^{[1
,2
]}

Huang, Tianxiang ^{[1
,2
]}

Kuwahara, Mizu ^{[1
,2
]}

Murakami, Akira ^{[1
,2
]}

机构：

[1] Juntendo Univ, Dept Ophthalmol, Grad Sch Med, Bunkyo Ku, Tokyo, Japan

[2] Juntendo Univ, Dept Digital Med, Grad Sch Med, Bunkyo Ku, Tokyo, Japan

[3] Juntendo Univ, Dept Hosp Adm, Grad Sch Med, Bunkyo Ku, Tokyo, Japan

[4] Juntendo Univ, Al Incubat Farm, Grad Sch Med, Bunkyo Ku, Tokyo, Japan

[5] Juntendo Univ, Dept Sports & Regenerat Med, Grad Sch Med, Bunkyo Ku, Tokyo, Japan

[6] Juntendo Univ, Dept Orthoped, Fac Med, Bunkyo Ku, Tokyo, Japan

[7] Juntendo Univ, Dept Med Technol Innovat Ctr, Bunkyo Ku, Tokyo, Japan

[8] Univ S Florida, Coll Med, Tampa, FL 33620 USA

来源：

BRITISH JOURNAL OF OPHTHALMOLOGY | 2024年 / 108卷 / 01期

关键词：

Cornea; Experimental; animal models; laboratory; Ocular surface; Wound healing; AUTOLOGOUS-SERUM; DRY EYE; EPITHELIAL DEFECT; CONTAMINATION; TEARS; RISK; PRP;

D O I：

10.1136/bjo-2022-322068

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Background/aims This study aimed to assess the efficacy and sterility of stored platelet-rich plasma (PRP) eye-drops for corneal epithelial wound healing compared with those of autologous serum (AS) eye-drops. Methods At our single institution, PRP and AS eye-drops were prepared using peripheral blood obtained from six healthy volunteers and stored at 4 degrees C. Platelet and leucocyte counts and transforming growth factor (TGF)-beta 1, epidermal growth factor (EGF), and fibronectin levels were assessed during storage for up to 4 weeks. Sterility was assessed by culturing 4-week poststorage samples. PRP, AS, and phosphate-buffered saline (PBS) eye-drop efficacies were compared using corneal epithelial wound healing assays in vitro and in vivo and monitoring wound areas under a microscope every 3 hours. Results Higher platelet and lower leucocyte counts were seen in PRP than in whole blood on the day of preparation. After storage, TGF-beta 1, EGF, and fibronectin levels were significantly higher in PRP than in AS eye-drops. In vitro and in vivo, PRP eye-drops used on the day of preparation significantly promoted corneal epithelial wound healing compared with PBS. Moreover, PRP eye-drops stored for 4 weeks significantly promoted corneal wound healing compared with PBS and AS eye-drops. Conclusion PRP eye-drops stored at 4 degrees C for 4 weeks promoted corneal epithelial wound healing with higher levels of growth factors than those observed in AS eye-drops, while maintaining sterility, suggesting that this preparation satisfies the unmet medical needs in the treatment of refractory keratoconjunctival epithelial disorders.

引用

页码：37 / 44

页数：8

共 49 条

[1] Sjogren's Syndrome: More Than Just Dry Eye [J].