Human polyomaviruses JCPyV and MCPyV in urothelial cell carcinoma: a single institution experience

被引：0

作者：

Klufah, Faisal ^{[1
,2
]}

Mobaraki, Ghalib ^{[1
,3
]}

Shi, Shuai ^{[1
]}

Marcelissen, Tom ^{[4
]}

Alharbi, Raed A. ^{[2
]}

Mobarki, Mousa ^{[5
]}

Almalki, Shaia Saleh R. ^{[2
]}

van Roermund, Joep ^{[4
]}

zur Hausen, Axel ^{[1
]}

Samarska, Iryna ^{[1
]}

机构：

[1] Maastricht Univ, GROW Sch Oncol & Reprod, Dept Pathol, Med Ctr, Maastricht, Netherlands

[2] Al Baha Univ, Fac Appl Med Sci, Dept Lab Med, Al Baha, Saudi Arabia

[3] Jazan Univ, Fac Appl Med Sci, Dept Med Lab Technol, Jazan, Saudi Arabia

[4] Maastricht Univ, Med Ctr, Dept Urol, Maastricht, Netherlands

[5] Jazan Univ, Fac Med, Pathol Dept, Jazan, Saudi Arabia

来源：

FRONTIERS IN ONCOLOGY | 2023年 / 13卷

关键词：

JCPyV; bladder cancer; polyomavirus; tumorigenesis; decoy cells; HPyV6; HPyV7; MCPyV; JC VIRUS; MERKEL CELL; BK VIRUS; URINE CYTOLOGY; DNA-SEQUENCES; DECOY CELLS; T-ANTIGEN; INFECTION; PAPILLOMAVIRUS; IDENTIFICATION;

D O I：

10.3389/fonc.2023.1251244

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ObjectiveUrothelial cell carcinoma (UCC) is the most common type of urinary bladder. JCPyV and BKPyV have been detected in the urine and tissue of urothelial cell carcinomas (UCC) in immunocompetent patients. Here, we investigated the presence of several HPyVs in UCC samples using diverse molecular techniques to study the prevalence of HPyVs in UCC.MethodsA large single-institution database of urine cytology specimens (UCS; n = 22.867 UCS) has previously been searched for decoy cells (n = 30), suggesting polyomavirus infection. The available urine sediments and formalin-fixed paraffin-embedded (FFPE) tissue samples of UCC patients were tested for the presence of JCPyV-LTAg expression by immunohistochemistry (IHC) labeled with SV40-LTAg antibody (clone: PAb416) and subsequent PCR followed by sequencing. In addition, the presence of the oncogenic Merkel cell polyomavirus (MCPyV) and the presence of human polyomavirus 6 (HPyV6) and 7 (HPyV7) DNA were tested with DNA PCR or IHC.ResultsOf the 30 patients harboring decoy cells, 14 were diagnosed with UCC of the urinary bladder (14/30; 46.6%) before presenting with decoy cells in the urine. The SV40-LTAg IHC was positive in all 14 UCC urine sediments and negative in the FFPE tissues. JCPyV-DNA was identified in all five available UCS and in three FFPE samples of UCC (three of 14; 21.4%). Two UCC cases were positive for MCPyV-DNA (two of 14; 14.3%), and one of them showed protein expression by IHC (one of 14; 7.1%). All specimens were HPyV6 and HPyV7 negative.ConclusionOur findings show the presence of JCPyV in the urine and UCC of immunocompetent patients. Moreover, MCPyV was detected in two UCC cases. In total, five UCC cases showed the presence of either JCPyV or MCPyV. The evidence here supports the hypothesis that these viruses might sporadically be associated with UCC. Further studies are needed to confirm the relevance of JCPyV or MCPyV as a possible risk factor for UCC development.

引用

页数：9

共 57 条

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