Development of an LC-MS/MS method for the determination of multiple mycotoxins in human urine

被引：0

作者：

Chen, Mobing ^{[1
,2
]}

Liu, Wei ^{[2
]}

Xiang, Ping ^{[2
]}

Xu, Linhao ^{[1
,2
]}

Tang, Yiling ^{[1
,2
]}

Kong, Ran ^{[2
]}

Yang, Shuo ^{[1
,2
]}

Yan, Hui ^{[2
]}

Di, Bin ^{[1
]}

机构：

[1] China Pharmaceut Univ, Sch Pharm, Nanjing, Peoples R China

[2] Acad Forens Sci, Shanghai Key Lab Forens Med, 1347 Guangfuxi Rd, Shanghai 200063, Peoples R China

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2023年 / 40卷 / 07期

关键词：

LC-MS; MS; mycotoxin; deoxynivalenol; zearalenone; aflatoxin B1; urine; QUANTITATIVE-DETERMINATION; DEOXYNIVALENOL EXPOSURE; ZEARALENONE; METABOLITES; BIOMARKERS; ADULTS;

D O I：

10.1080/19440049.2023.2222842

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A sensitive liquid chromatography-tandem mass spectrometry method was developed for the determination of 17 mycotoxins in human urine. The method incorporates a two-step liquid-liquid extraction with ethyl acetate:acetonitrile (7:1), which had good extraction recovery. The LOQs of all mycotoxins ranged from 0.1 to 1 ng/mL. Intra-day accuracy ranged from 94 to 106%, and intra-day precision ranged from 1 to 12% for all mycotoxins. Inter-day accuracy and precision were 95-105% and 2-8%, respectively. The method was successfully applied to investigate the urine levels of 17 mycotoxins from 42 volunteers. Deoxynivalenol (DON, 0.97-9.88 ng/mL) was detected in 10 (24%) urine samples and zearalenone (ZEN, 0.13-1.11 ng/mL) in 2 (5%) urine samples.

引用

页码：917 / 927

页数：11

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