Effects of ?-carrageenan addition and chlorogenic acid covalent crosslinking on protein conformation and gelling properties of soy protein hydrogels

被引:27
作者
Man, Hui
Jia, Yijia [1 ]
Song, Hanyu [1 ]
Yan, Xinyue
Zhang, Dongmeng [1 ]
Huang, Yuyang [2 ]
Qi, Baokun [1 ]
Zhang, Shuang [1 ]
Li, Yang [1 ,3 ,4 ]
机构
[1] Northeast Agr Univ, Coll Food Sci, Harbin 150030, Heilongjiang, Peoples R China
[2] Harbin Univ Commerce, Coll Food Engn, Harbin 150028, Heilongjiang, Peoples R China
[3] Northeast Agr Univ, Natl Res Ctr Soybean Engn & Technol, Harbin 150028, Peoples R China
[4] Northeast Agr Univ, Heilongjiang Green Food Sci Res Inst, Harbin 150028, Peoples R China
关键词
Chlorogenic acid; Covalent crosslinking; Soy protein isolate; -carrageenan; Hydrogel; KAPPA-CARRAGEENAN; GEL PROPERTIES; GELATIN GELS; BEHAVIOR;
D O I
10.1016/j.lwt.2023.114434
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
This paper reports the effects of kappa-carrageenan (KC) additions (0.6% w/v) and covalent crosslinking by chlorogenic acid (CA) on the protein conformation and gelling properties of soybean protein isolate (SPI) hydrogels. Treatment with KC and CA changed the conformation of the SPI and improved the antioxidant ca-pacity and gel properties, as confirmed by fluorescence spectroscopy, Fourier-transform infrared spectroscopy, antioxidant capacity analysis, and rheology measurements. The addition of CA (0.01 g/g-SPI) decreased the number of free amino groups, free sulfhydryl content, tryptophan fluorescence intensity, and surface hydro-phobicity of SPI by 44.22%, 10.05%, 30.47%, and 44.61%, respectively, and increased the DPPH and ABTS radical scavenging activities by 30.29% and 40.69%, respectively. The addition of KC (0.6% w/v) enhanced the DPPH radical scavenging activities of the SPI-CA (0.002 and 0.01 g/g-SPI) conjugates by 11.29-16.50%, and improved the viscoelasticity, water immobilization, density, and thermal stability of the SPI-CA gels.
引用
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页数:10
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