Fungal strain improvement for efficient cellulase production and lignocellulosic biorefinery: Current status and future prospects

被引：26

作者：

Yang, Jie ^{[1
]}

Yue, Hou-Ru ^{[1
]}

Pan, Li-Ya ^{[2
]}

Feng, Jia-Xun ^{[2
]}

Zhao, Shuai ^{[2
]}

Suwannarangsee, Surisa ^{[3
]}

Champreda, Verawat ^{[3
]}

Liu, Chen-Guang ^{[1
]}

Zhao, Xin-Qing ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

[2] Guangxi Univ, Coll Life Sci & Technol, Guangxi Res Ctr Microbial & Enzyme Engn Technol, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530004, Peoples R China

[3] Natl Ctr Genet Engn & Biotechnol BIOTEC, 113 Thailand Sci Pk,Phaholyothin Rd,Khlong Luang, Pathum Thani 12120, Thailand

来源：

BIORESOURCE TECHNOLOGY | 2023年 / 385卷

关键词：

Lignocellulosic biomass; Cellulase; Filamentous fungi; Metabolic engineering; Omics approaches; TRICHODERMA-REESEI RUT-C30; RNAI-MEDIATED GENE; CELL FACTORIES; IN-VIVO; TRANSCRIPTION; PROMOTER; PROTEIN; OVEREXPRESSION; REPRESSION; DISCOVERY;

D O I：

10.1016/j.biortech.2023.129449

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Lignocellulosic biomass (LCB) has been recognized as a valuable carbon source for the sustainable production of biofuels and value-added biochemicals. Crude enzymes produced by fungal cell factories benefit economic LCB degradation. However, high enzyme production cost remains a great challenge. Filamentous fungi have been widely used to produce cellulolytic enzymes. Metabolic engineering of fungi contributes to efficient cellulase production for LCB biorefinery. Here the latest progress in utilizing fungal cell factories for cellulase production was summarized, including developing genome engineering tools to improve the efficiency of fungal cell factories, manipulating promoters, and modulating transcription factors. Multi-omics analysis of fungi contributes to identifying novel genetic elements for enhancing cellulase production. Furthermore, the importance of translation regulation of cellulase production are emphasized. Efficient development of fungal cell factories based on integrative strain engineering would benefit the overall bioconversion efficacy of LCB for sustainable bioproduction.

引用

页数：11