CYP6CX2 and CYP6CX3 mediate thiamethoxam resistance in field whitefly, Bemisia tabaci (Hemiptera:Aleyrodidae)

被引：6

作者：

Yang, Jing ^{[1
]}

Fu, Buli ^{[1
]}

Gong, Peipan ^{[1
]}

Zhang, Chengjia ^{[1
]}

Wei, Xuegao ^{[1
]}

Yin, Cheng ^{[1
]}

Huang, Mingjiao ^{[1
]}

He, Chao ^{[1
]}

Du, Tianhua ^{[1
]}

Liang, Jinjin ^{[1
]}

Liu, Shaonan ^{[1
]}

Ji, Yao ^{[1
]}

Xue, Hu ^{[1
]}

Wang, Chao ^{[1
]}

Hu, Jinyu ^{[1
]}

Du, He ^{[1
]}

Zhang, Rong ^{[1
]}

Yang, Xin ^{[1
]}

Zhang, Youjun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

来源：

JOURNAL OF ECONOMIC ENTOMOLOGY | 2023年 / 116卷 / 04期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

cytochrome P450 monooxygenase; thiamethoxam; neonicotinoid; insecticide resistance; INSECTICIDE RESISTANCE; BROWN PLANTHOPPER; CYTOCHROME-P450; MONOOXYGENASE; Q-BIOTYPE; HEMIPTERA; NEONICOTINOIDS; DETOXIFICATION; EXPRESSION; OVEREXPRESSION; MECHANISMS;

D O I：

10.1093/jee/toad089

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Cytochrome P450 monooxygenases (P450s) are well-known for their crucial roles in the detoxification of xenobiotics. However, whether CYP6CX2 and CYP6CX3, 2 genes from our Bemisia tabaci (B. tabaci) MED/Q genome data were associated with detoxification metabolism and confer resistance to thiamethoxam is unclear. In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that mRNA levels of CYP6CX2 and CYP6CX3 were up-regulated after exposure to thiamethoxam. Transcriptional levels of 2 genes were overexpressed in laboratory and field thiamethoxam resistant strains by RT-qPCR. These results indicate that the enhanced expression of CYP6CX2 and CYP6CX3 appears to confer thiamethoxam resistance in B. tabaci. Moreover, linear regression analysis showed that the expression levels of CYP6CX2 and CYP6CX3 were positively correlated with thiamethoxam resistance levels among populations. The susceptibility of whitefly adults was markedly increased after silencing 2 genes by RNA interference (RNAi) which further confirming their major role in thiamethoxam resistance. Our findings provide information to better understand the roles of P450s in resistance to neonicotinoids and suggest that these genes may be applied to develop target genes for sustainable management tactic of agricultural pests such as B. tabaci.

引用

页码：1342 / 1351

页数：10

共 24 条

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[22] Assessment of the Bemisia tabaci CYP6CM1vQ transcript and protein levels in laboratory and field-derived imidacloprid-resistant insects and cross-metabolism potential of the recombinant enzyme
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[23] Positional cloning of rp2 QTL associates the P450 genes CYP6Z1, CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus
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[24] Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance in Laodelphax striatellus (Fallen)
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