Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

被引:3
作者
Aota, Illia [1 ,2 ]
Yatsuda, Chisato [1 ]
Izawa, Ei-ichi [1 ]
机构
[1] Keio Univ, Dept Psychol, 2-15-45 Mita, Tokyo 1088345, Japan
[2] Japan Soc Promot Sci, Tokyo, Japan
关键词
bird; corticosterone; enzyme-linked immunoassay; glucocorticoid; stress; STRESS; CORTISOL; HORMONES; GLUCOCORTICOIDS; CHALLENGE; RESPONSES; ACTH;
D O I
10.1292/jvms.22-0383
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal's stress or metabolic state, commonly used in mammals. However, the validity of salivarycorticosterone (CORT) measurements in birds has notyet been reported. We aimed to measure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10-60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.
引用
收藏
页码:71 / 75
页数:5
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