Association of Angiopoietin-Like Protein-8 Gene Variant (Rs2278426 (C/T)) in a Cohort of Egyptian Patients with Metabolic Syndrome: A Case-Control Study

Objective: This study was conducted to reveal the asso-ciation of ANGPTL8 gene variant (Rs2278426 (C/T)) with metabolic syndrome in a cohort of Egyptian patients. Materials and methods: This study is a case control study that included 150 patients with metabolic syn-drome and 150 healthy control subjects. All subjects were submitted to history taking and thorough physi-cal examination and laboratory analysis. Genomic DNA was extracted and ANGPTL8 gene SNP (rs2278426) was detected by 5' nuclease assay. The tested genotypes included homozygous genotypes for C allele (CC), ho-mozygous genotypes for T allele (TT) and heterozygous genotypes (CT). Results: A total of 300 subjects were included in the study; group 1 included 150 patients with metabolic syndrome. 21.3% were males and 78.7% were females, and group 2 had 150 healthy subjects. 17.3% were males, and 82.7% were females. In the current study, the metabolic syndrome group showed dysregulation of lipids and fasting plasma glucose (FPG) with a sta-tistically significant increase in body anthropometric measures. There was no statistically significant differ- ence in the distribution of the heterozygous genotype (C/T) relative to the wild-type genotype (C/C) in each of the two tested groups (p = 0.287 and 0.245 in the metabolic syndrome and control groups, respectively). Conclusions: There was no statistically significant dif- ference in the genotype distribution of ANGPTL8 gene variant (Rs2278426) genotypes between the metabolic syndrome and control groups. The homozygous reces-sive genotype for T allele (TT) was not detected in both tested (Clin Diabetol 2023; 12; 4: 215-222)