Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling

被引：4

作者：

Kasmi, Yassine ^{[1
]}

Blancke, Tina ^{[1
]}

Eschbach, Erik ^{[1
]}

Moeckel, Benita ^{[1
]}

Casas, Laura ^{[2
]}

Bernreuther, Matthias ^{[3
]}

Nogueira, Pedro ^{[1
]}

Delfs, Gertrud ^{[3
]}

Kadhim, Samira ^{[3
]}

Meissner, Timo ^{[3
]}

Roediger, Marcellus ^{[3
]}

Eladdadi, Amina ^{[4
,5
]}

Stransky, Christoph ^{[3
]}

Hanel, Reinhold ^{[1
]}

机构：

[1] Thunen Inst Fisheries Ecol, Bremerhaven, Germany

[2] CSIC, Inst Invest Marinas IIM, Inst Marine Res, Vigo, Spain

[3] Thunen Inst Sea Fisheries, Bremerhaven, Germany

[4] Coll St Rose, Dept Math, Albany, NY USA

[5] Natl Sci Fdn, Div Math Sci, Alexandria, VA USA

来源：

FRONTIERS IN MARINE SCIENCE | 2023年 / 10卷

基金：

美国国家科学基金会;

关键词：

Environmental DNA (eDNA); quantitative eDNA analysis; bottom trawl sampling; Gadus morhua; North Sea; Baltic Sea; eDNA modeling; FISHERIES; EDNA;

D O I：

10.3389/fmars.2023.1058354

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The assessment of fish stocks is often dependent on scientific trawl fisheries surveys, which are both invasive and costly. The analysis of environmental DNA (eDNA) from water samples is regarded as a non-invasive and cost-effective alternative, but meaningful performance evaluations are required for a wider application. The goal of this study was to comparatively analyze a newly developed, more sensitive real-time PCR based eDNA approach with bottom trawl fisheries catches to locally detect and quantify Atlantic cod (Gadus morhua) in the North and Baltic Seas. With a species-specificity of the qPCR assay of 100%, a minimal limit of 15 Cytochrome b eDNA copies was determined for the detection of cod. In addition, a Gaussian processing regression proved a significant correlation (95%) between eDNA (copies per L of water) and cod biomass (CPUE/Ha) found by bottom trawling. The results presented here prove the potential of eDNA analyses for quantitative assessments of commercial fish stocks in the open ocean, although additional comparative analyses are needed to demonstrate its performance under different oceanographic conditions.

引用

页数：14

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