Biogenic Synthesis of Copper Oxide Nanoparticles Using Pimpinella anisum Seed Extract Characterization and Antibacterial activity

Recent attention has been drawn to green methods for preparing metal oxide nanoparticles due to their ease of preparation and environmental friendliness in comparison with chemical and physical methods. By using an Eco-friendly reducing agent, Pimpinella anisum seed extract, this study describes a method for synthesizing CuO nanoparticles that are based on biological principles. Copper oxide nanoparticles were produced utilizing a precipitation technique involving Pimpinella anisum extract, (CuCl2.2H2O), and (NaOH). To determine the morphology of CuONPs and their elemental composition, shape, size, and type of bonds present in the sample, UV-Vis spectrophotometry, XRD, SEM, and EDS analysis have all been performed. Spectra of the UV-Visible spectrum showed an absorption peak between 250-300nm, which confirms the formation of CuO nanoparticles. FT-IR spectral showed bands related to CuO vibration at 529 cm-1 are, suggesting the production of CuO nanoparticles. Analysis by X-ray diffraction revealed that the CuO nanoparticles produced were monoclinic in phase and had mean size of 22nm. Based on SEM analysis, the obtained nanoparticles have an aspherical shape. EDX analysis was used to confirm copper and oxygen presence. The weight percentage of the latter was (71.2% Cu and 28.8% O), respectively. Four pathogenic microorganisms were used to evaluate the antibacterial efficacy of green-synthesized CuO nanoparticles. As a result, the CuONPs prepared to have the potential for application as antimicrobial agents, suggesting that they may be useful for therapeutic applications in e future.