Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury

被引:2
作者
Shen, Weiyun [1 ,2 ]
Liu, Shuxin [1 ,2 ]
Wei, Xiaojing [1 ,2 ]
Wang, Yaping [1 ,2 ,3 ]
Yang, Lin [1 ,2 ]
机构
[1] Cent South Univ, Xiangya Hosp 2, Dept Anesthesiol, Changsha 41000, Hunan, Peoples R China
[2] Cent South Univ, Hunan Prov Ctr Clin Anesthesia & Anesthesiol, Res Inst, Changsha 41000, Hunan, Peoples R China
[3] Cent South Univ, Xiangya Hosp 2, Dept Pain Management, Changsha 41000, Peoples R China
基金
中国国家自然科学基金;
关键词
Bone morphogenetic protein 4; Monocyte; Monocyte-derived macrophage; Spinal cord injury; Blood spinal cord barrier; BONE MORPHOGENETIC PROTEIN-4; PRECURSOR CELLS; BRAIN-INJURY; MICROGLIA; NOGGIN; POLARIZATION; DIFFERENTIATION; MACROPHAGES; ACTIVATION; EXPRESSION;
D O I
10.1242/dmm.049856
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Bone morphogenetic protein (BMP)4 plays a critical role in regulating neuronal and glial activity in the course of spinal cord injury (SCI). The underlying cause and cellular source of BMP4 accumulation at the injured spinal cord remain unclear. Here, we observed that plasma BMP4 levels are statistically higher in SCI patients than in healthy donors. When comparing rats in the sham group (T9 laminectomy without SCI) with rats in the SCI group, we found a persistent decline in BBB scores, together with necrosis and mononuclear cell accumulation at the contusion site. Moreover, during 2 weeks after SCI both plasma and cerebrospinal fluid levels of BMP4 displayed notable elevation, and a positive correlation. Importantly, percentages of circulating BMP4-positive (BMP4+) monocytes and infiltrating MDMs were higher in the SCI group than in the sham group. Finally, in the SCI+clodronate liposome group, depletion of monocytes effectively attenuated the accumulation of both BMP4+ MDMs and BMP4 in the injured spinal cord. Our results indicated that, following SCI, infiltrating MDMs provide an important source of BMP4 in the injured spinal cord and, therefore, might serve as a potential therapeutic target.
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页数:12
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共 71 条
[1]   Peripheral blood monocyte count at onset may affect the prognosis in multiple sclerosis [J].
Akaishi, Tetsuya ;
Takahashi, Toshiyuki ;
Nakashima, Ichiro .
JOURNAL OF NEUROIMMUNOLOGY, 2018, 319 :37-40
[2]  
Al-Sammarraie Nadia, 2021, Neuroimmunol Neuroinflamm, V8, P53, DOI 10.20517/2347-8659.2020.34
[3]   Bone morphogenetic proteins 4, 6, and 7 are up-regulated in mouse spinal cord during experimental autoimmune encephalomyelitis [J].
Ara, Jahan ;
See, Jill ;
Mamontov, Polina ;
Hahn, Ashleigh ;
Bannerman, Peter ;
Pleasure, David ;
Grinspan, Judith B. .
JOURNAL OF NEUROSCIENCE RESEARCH, 2008, 86 (01) :125-135
[4]   Endothelial BMP4 Promotes Leukocyte Rolling and Adhesion and Is Elevated in Patients After Survived Out-of-Hospital Cardiac Arrest [J].
Arnold, Linus ;
Weberbauer, Miki ;
Herkel, Marius ;
Fink, Katrin ;
Busch, Hans-Jorg ;
Diehl, Philipp ;
Grundmann, Sebastian ;
Bode, Christoph ;
Elsasser, Albrecht ;
Moser, Martin ;
Helbing, Thomas .
INFLAMMATION, 2020, 43 (06) :2379-2391
[5]   Blockade of CD73 delays glioblastoma growth by modulating the immune environment [J].
Azambuja, J. H. ;
Schuh, R. S. ;
Michels, L. R. ;
Iser, I. C. ;
Beckenkamp, L. R. ;
Roliano, G. G. ;
Lenz, G. S. ;
Scholl, J. N. ;
Sevigny, J. ;
Wink, M. R. ;
Stefani, M. A. ;
Battastini, A. M. O. ;
Figueiro, F. ;
Teixeira, H. F. ;
Braganhol, E. .
CANCER IMMUNOLOGY IMMUNOTHERAPY, 2020, 69 (09) :1801-1812
[6]   Emerging Role of Bone Morphogenetic Protein 4 in Metabolic Disorders [J].
Baboota, Ritesh K. ;
Blueher, Matthias ;
Smith, Ulf .
DIABETES, 2021, 70 (02) :303-312
[7]   Serum neurofilament as a predictor of disease worsening and brain and spinal cord atrophy in multiple sclerosis [J].
Barro, Christian ;
Benkert, Pascal ;
Disanto, Giulio ;
Tsagkas, Charidimos ;
Amann, Michael ;
Naegelin, Yvonne ;
Leppert, David ;
Gobbi, Claudio ;
Granziera, Cristina ;
Yaldizli, Ozgur ;
Michalak, Zuzanna ;
Wuerfel, Jens ;
Kappos, Ludwig ;
Parmar, Katrin ;
Kuhle, Jens .
BRAIN, 2018, 141 :2382-2391
[8]   Clinical, Radiological, and Laboratory Features of Spinal Cord Involvement in Primary Sjogren's Syndrome [J].
Butryn, Michaela ;
Neumann, Jens ;
Rolfes, Leoni ;
Bartels, Claudius ;
Wattjes, Mike P. ;
Mahmoudi, Nima ;
Seeliger, Tabea ;
Konen, Franz F. ;
Thiele, Thea ;
Witte, Torsten ;
Meuth, Sven G. ;
Skripuletz, Thomas ;
Pawlitzki, Marc .
JOURNAL OF CLINICAL MEDICINE, 2020, 9 (05)
[9]   Bone Morphogenetic Protein 4 Signalling in Neural Stem and Progenitor Cells during Development and after Injury [J].
Cole, Alistair E. ;
Murray, Simon S. ;
Xiao, Junhua .
STEM CELLS INTERNATIONAL, 2016, 2016
[10]   Expression of Bone Morphogenetic Proteins in Multiple Sclerosis Lesions [J].
Costa, Carme ;
Eixarch, Herena ;
Martinez-Saez, Elena ;
Calvo-Barreiro, Laura ;
Calucho, Maite ;
Castro, Zoraida ;
Ortega-Aznar, Arantxa ;
Ramon y Cajal, Santiago ;
Montalban, Xavier ;
Espejo, Carmen .
AMERICAN JOURNAL OF PATHOLOGY, 2019, 189 (03) :665-676